*2019년 이후 취급 하지 않음

DNATerminator® End Repair Kit

Repair sheared or restriction-digested DNA ends
Increase cloning efficiency as much as a 500%
Optimized system for consistent & reliable results

Repair DNA ends

Double-stranded DNA fragments for library construction or large-scale sequencing are often generated by mechanically shearing larger (genomic) DNA, a process that primarily leaves uneven ends. Large DNA may also be cut with restriction enzymes to generate smaller fragments for subcloning. Sheared or restricted DNA fragments must be “end-repaired” before ligation into blunt-end cloning vectors (e.g., Lucigen’s CloneSmart®, CopyRight®, or BigEasy® systems).

The DNATerminator End Repair Kit creates blunt, 5' phosphorylated ends on any type of sheared or restriction-digested DNA fragment, ensuring the highest possible cloning efficiency (Figure 1).

Figure 1. Effect of DNA shearing and end-repair methods on library construction efficiency. Shotgun libraries were constructed using 250 ng of lambda DNA sheared by sonication (Son) or by the GeneMachines® HydroShear™ device (HS). Sheared DNA was repaired with T4 DNA polymerase and Klenow fragment (TK) or DNATerminator End Repair Kit (DT) and cloned using Lucigen’s CloneSmart® system. Values are colony forming units (cfu) per library.

MA002-DNA-Terminator.pdf (200,977kb)

Means_End.pdf (230,101kb)

MSDS.pdf (118,131kb)