CloneDirect¢â Rapid Ligation Kit

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CloneDirect¢â Rapid Ligation Kit

  • Ligation reaction is transformation-ready in minutes
  • No DNA cleanup step required
  • Highly reliable and reproducible results

Quicken cloning

The CloneDirect Rapid Ligation Kit is a simple, rapid, highly efficient alternative to standard ligation and transformation protocols. This kit eliminates the requirement for a post-ligation cleanup step before electroporation. A 15-minute heat denaturation at 70¡ÆC is all that is needed prior to cell transformation.
 
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Figure 1. Ligation efficiencies measured by transformation. Ligation reactions were heat denatured at 70¡ÆC and used without additional cleanup to transform electrocompetent E. cloni¢ç 10G cells. Transformants (in millions) are shown.
 
 
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