*2019³â ÀÌÈÄ Ãë±Þ ÇÏÁö ¾ÊÀ½CloneDirect¢â Rapid Ligation Kit
Quicken cloningThe CloneDirect Rapid Ligation Kit is a simple, rapid, highly efficient alternative to standard ligation and transformation protocols. This kit eliminates the requirement for a post-ligation cleanup step before electroporation. A 15-minute heat denaturation at 70¡ÆC is all that is needed prior to cell transformation.
Figure 1. Ligation efficiencies measured by transformation. Ligation reactions were heat denatured at 70¡ÆC and used without additional cleanup to transform electrocompetent E. cloni¢ç 10G cells. Transformants (in millions) are shown.
MA003-CloneDirect-Rapid-Ligation-Kit.pdf (197,133kb) MSDS.pdf (118,131kb) |
»ç¿ëÈıâ ÀÛ¼º
|