The Oris™ Pro Cell Migration Assay enables the use of automated liquid handling equipment for cell seeding and allows unrestricted access to cells throughout the experiment. The Oris™ Pro Assay uses a non-toxic biocompatible gel, BCG (see Figure 1: Well Bottom with BCG), to form a cell-free zone on culture surfaces. After seeding cells into the 96-well plate, the BCG dissolves permitting cells to migrate into the well centers (see Figure 2: Assay Schematic). Oris™ Pro Assays* are available with Tissue Culture Treated or Collagen I Coated surfaces. Cells can be stained and the cells in the Detection Zone can be captured and quantifed in real-time using microscopes and High Content Screening (HCS)/High Content Imaging (HCI) instruments. Staining live cells at the beginning of the experiment permits real-time images to be captured at any time point. Both endpoint and kinetic data can be generated.
Features & Benefits of the Oris™ Pro Cell Migration Assay
- Increased Productivity - Treat cells with multiple fluorescent probes, labels, or colorimetric stains for multi-parametric measurements with microscopes, High Content Screening (HCS) and High Content Imaging (HCI) instruments.
- Less Handling - Realize reduced assay handling time with an assay format in which a centrally placed biocompatible gel automatically dissolves to reveal a detection zone.
- Automation-Friendly Design - Utilize automated liquid handling equipment for fast set-up of high throughput, 96-well assays.
- Reproducible Results - Achieve well-to-well CV's <12% and generate robust Z factors suitable for compound screening.
- Real-Time Analysis - Experience unlimited access to cells, cell morphology and cell movement from cell seeding to assay readout.
Representative Data
Figure 3: Oris™ Pro Cell Migration Analysis: HUVECs were seeded onto an Oris™ Pro Collagen I plate. A dose-response titration was performed using the actin polymerization inhibitor, Cytochalasin D. Cells were treated for 18 hours, fixed, and stained for F-actin. Images were acquired using the BD Pathway™ 855 Bioimaging System and a dose-response curve with IC50 was generated (n=16).
* Patent Pending.
Oris™ is a trademark of Platypus Technologies, LLC
BD Pathway™ 855 Bioimaging System is a registered trademark of BD Biosciences.
IDT
