VELOCITY DNA polymerase is a fast thermostable enzyme possessing 5¢¥-3¢¥ and 3¢¥-5¢¥ proofreading exonuclease activities. VELOCITY provides high-fidelity, with an error-rate of 4.4 x 10-7, combined with a high speed DNA synthesis as fast as 15s/kb for templates of up to 5kb and 30s/kb for templates longer than 5kb.
Features & Benefits• ³ôÀº ¼Óµµ, °í¼º´ÉÀÇ DNA polymerase
• ³ôÀº ¹ÝÀÀ ÁøÇ༺ (high-processivity) • ºü¸¥ ÁõÆø • ±ä templateÀ¸·Î ÁõÆø ½Ã ½Ã°£ ´ÜÃà • ¹ÝÀÀ ½ÃÀÛ Àü ÃÖÀûÈ ½ÃÄÑ¾ß ÇÒ Á¶°ÇÀÌ ÀûÀ½ Applications• GC-rich templates• °í¼º´ÉÀÌ ÇÊ¿äÇÑ cloning • Blunt-end cloning • ÁõÆøÀÌ ¾î·Á¿î templates • Site directed mutagenesis DescriptionVELOCITY DNA polymerase´Â ÃÊ°í¼ÓÀ¸·Î ºü¸¥ ³»¿¼º enzymeÀ¸·Î 3'-5' proofreading exonuclease activitiy¸¦ °®°í ÀÖ½À´Ï´Ù. VELOCITY enzymeÀº ¾ÆÁÖ ÀÛÀº ¾çÀÇ target¸¸ °¡Áö°íµµ ¶Ù¾î³ PCR yield¸¦ Á¦°øÇϸç fidelity ¿ª½Ã ¿ì¼öÇÕ´Ï´Ù. ¶ÇÇÑ ÀÌ enzymeÀº ºü¸¥ ¹ÝÀÀÁøÇ༺ Ư¡À» °¡Áö°í ÀÖ¾î extension ½Ã°£ÀÌ Âª¾Æ ±ä tartgetÀ» ºü¸¥ ¼Óµµ¿Í ³ôÀº ¼öÀ²·Î ÁõÆøÀÌ °¡´ÉÇÕ´Ï´Ù. TemplateÀÇ purity°¡ ¶³¾îÁö°Å³ª º¹ÀâÇÑ targetÀ» »ç¿ëÇÏ´õ¶óµµ ¼öÀ²ÀÌ ³ôÀ¸¸é¼ ¾çÁúÀÇ »ê¹°À» Á¦°ø ÇÕ´Ï´Ù. VELOCITY´Â ³ôÀº fidelity¸¦ Á¦°øÇÏ¸é¼ ¸Å¿ì ³·Àº error-rate(4.4X10-7)À» Á¦°øÇÕ´Ï´Ù. Processivity°¡ ³ô¾Æ ÃÖ´ë 5kb Å©±âÀÇ targetÀº ÃÖ¼Ò 15s/kbÀÇ ¼Óµµ·Î extensionÀÌ °¡´ÉÇÏ°í 5 kb ÀÌ»óÀÇ targetÀÏ °æ¿ì¿£ 30s/kbÀÇ ¼Óµµ·Î extensionÀÌ °¡´ÉÇÕ´Ï´Ù. ±ä PCR product¸¦ ³ôÀº ¼öÀ²·Î mutationÀÌ ÀϾÁö ¾Ê°Ô ÁõÆøÇÏ°í ½Í´Ù¸é VELOCITY°¡ °¡Àå Ź¿ùÇÑ ¼±ÅÃÀÔ´Ï´Ù. Concentration2u/¥ìl Components
Storage & StabilityAll components should be stored at -20¡ÆC upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label. Shipping conditionsOn Dry Ice or Blue Ice. protocol [VELOCITY_DNA_Polymerase_protocol.pdf] (137,700kb) Fact sheet [VELOCITY_DNA_Polymerase.pdf] (238,747kb) |
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