Lentiviral vectors provide effective vehicles for delivery of genetic content into a wide range of cell types including both dividing and non-dividing or post-mitotic cells.  Lentiviral vectors have the ability to transduce cells and integrate into the host genome resulting in stable, long-term expression of the transgene both in vivo and in vitro.

Lentiviral particles are generated using standardized methods. HEK293 cells are co-transfected  with the lentiviral expression vector and packaging plasmids to express the necessary components for virus assembly.  Mature viral particles bud from the cell and into the supernatant. Supernatant can be harvested and applied directly to target cells or concentrated.

Using optimized in-house protocols and reagents, transOMIC technologies has the capability and expertise to provide top-quality, reliable lentiviral particles expressing shRNAs. Lentiviral stocks of individual shRNA clones, sets targeting one or more genes, genome-scale shRNA pools, and pools targeting specific gene families are available.  Using our custom, on-demand services, custom shRNA pools can also be generated from a customer provided gene list. 

Lentiviral particles are generated using standardized methods, which includes co-transfection of the lentiviral expression vector with lentiviral packaging plasmids into 293T cells, harvesting of the virus-containing supernatant, and concentration of the viral particles.
Lentiviral titers are determined using methods that measure functional expression in transduced cells (such as fluorescent marker expression or antibiotic selection), and not simply the physical presence of viral particle components.  This provides higher assurance of the quality of the viral stocks leading to more consistent and reliable results.

transOMIC technologies provides customers with maximal flexibility to meet the requirements of the target cell type or experimental design.  Genetic content can be cloned into currently available lentiviral vectors, custom vectors developed for customers or customer provided vectors, and viral particles can be produced at various titers and volumes.

Standard packaging can be easily ordered.  Use the gene search below to identify constructs then add the selected service to your cart. 

Catalog Number	Product (Packaged virus)	Titer (TU/ml)	Volume
TSE0003	Target Gene Set: 3-4 shRNA targeting the gene of interest plus non-targeting controls	> 106	1 ml 5 x 200 µl
TSE0001	Target Gene Set: 3-4 shRNA targeting the gene of interest plus non-targeting controls	> 108	100 µl 4 x 25 µl
TSE0011	Starter Kit: 3-4 shRNA targeting the gene of interest plus positive and non-targeting controls	> 108	100 µl 4 x 25 µl

      Turnaround time  is ~4 weeks