IMMOLASE¢â DNA Polymerase
CAT# PRODUCT SIZE STORAGE ¼ÒºñÀÚ°¡ ÁÖ¹®
BIO-21047IMMOLASE DNA Polymerase500 UNITS-20¡ÆC-·Î±×ÀÎ
BIO-21048IMMOLASE DNA Polymerase5000 UNITS-20¡ÆC-·Î±×ÀÎ
-·Î±×ÀÎ
immolase.jpg
 IMMOLASE¢â is a heat-activated, thermostable DNA polymerase. IMMOLASE provides high yield and improved specificity when compared to standard polymerases and can eliminate the presence of non-specific binding, such as primer-dimers and mis-primed products.




 

Features & Benefits

• Heat-activated ³»¿­¼ºÀÇ DNA polymerase
• ¶Ù¾î³ª°í °ß°íÇÑ performance
• ¾çÀûÀÎ assay ¼öÇà ½Ã ÈǸ¢ÇÑ yield º¸ÀÓ
• »ó¿Â¿¡¼­µµ ½ÇÇè ÇÒ ¼ö ÀÖ´Â Æí¸®¼º
• TA cloning °¡´É ('A' overhang)

 

Applications

• Multiplex PCR reaction ƯÈ÷ ³ôÀº ƯÀ̼º
• TA cloning
• ¾ÆÁÖ ÀûÀº ¾çÀÇ template
• Multiple PCR reaction °¡´É

Description

IMMOLASE¢â ´Â heat-activated ÇÑ ³»¿­¼º DNA polymeraseÀÔ´Ï´Ù. ³ôÀº ¼öÀ²ÀÇ »ê¹°À» Á¦°øÇϸç (Fig.1) ÀÏ¹Ý polymerase¿Í
ºñ±³ÇßÀ» ¶§ °¨µµ¸é¿¡¼­ ¿ùµîÈ÷ ¶Ù¾î³³´Ï´Ù. ±×¸®°í primer-dimer³ª miss-primed °á°ú¹° µî ºñƯÀÌÀûÀÎ °áÇÕÀ» ÇÏÁö ¾Êµµ·Ï Çϴ Ư¡À» °¡Áö°í ÀÖ½À´Ï´Ù. º¸Åë »ó¿Â¿¡¼­´Â enzymeÀÇ È°¼ºÀÌ ¾ø±â ¶§¹®¿¡ PCR ½ÃÀÛ Àü pre-heating °úÁ¤À» 10ºÐ°£ ÁøÇàÀ» ÇØ¾ß ÇÕ´Ï´Ù.  ÀÌ pre-heating °úÁ¤À» ÁøÇàÇÏ¸ç ½ÇÇèÀÚ´Â PCR reagentsµéÀ» ¼¯´Â °úÁ¤À» µ¿½Ã¿¡ ÁøÇàÇÒ ¼ö Àֱ⠶§¹®¿¡ setup ½Ã°£À» µû·Î ÀâÁö ¾Ê¾Æµµ µÇ¾î Æí¸®ÇÕ´Ï´Ù. °Ô´Ù°¡ ±âÁ¸ÀÇ ´Ù¸¥ ³»¿­¼º DNA polymeraseÀÇ protocolÀ» °¡Áö°í IMMOLASE¢â·Î ½ÇÇè °¡´ÉÇϱ⠶§¹®¿¡ Á¶°ÇÀ» µû·Î ÀâÀ» ¹ø°Å·Î¿òÀÌ ÁÙ¾îµì´Ï´Ù. ¶ÇÇÑ 2X PolyMate Additive(BIO-37041)¸¦ Ãß°¡·Î »ç¿ëÇÏ¿© IMMOLASE¢âÀÇ °¨µµ¿Í ¼º´ÉÀ» ¶Ç Çѹø ³ôÀÏ ¼ö ÀÖ½À´Ï´Ù. ƯÈ÷GC- ³ª AT-°¡ ¸¹Àº DNA sequence³ª secondary structure°¡ ¸¹Àº template¿¡ ¾ÆÁÖ È¿°úÀûÀÔ´Ï´Ù.

Notes

Immolase is a trademark of Bioline.

Components


Product
250 Units
500 Units
5000 Units
IMMOLASE DNA Polymerase
1 x 50¥ìl
1 x 100¥ìl
10 x 100¥ìl
10x ImmoBuffer
 1.2ml
2 x 1.2ml
20 x 1.2ml
50mM MgCl2 Solution
 1.2ml
2 x 1.2ml
20 x 1.2ml

Concentration

5u/¥ìl

Storage & Stability

All components should be stored at -20¡ÆC upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

Shipping conditions

Shipped on Dry Ice or Blue Ice.

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