Protein:Protein Interaction (PPI) Analysis

StarGate® for Bait Cloning

This novel cloning system is the perfect tool for efficient screening and fast identification of the optimal tag for PPI investigation with a given bait. Once the bait protein is cloned into the Donor Vector, a large selection of Acceptor Vectors for its expression with different tag arrangements is available.

For PPI analysis we provide Cloning Kits ( with different Acceptor Vectors) consisting of all required Products to perform the entire StarGate® cloning procedure to generate the desired bait expression vector.

All Kits consist of

StarGate® Combi Entry Cloning Set Cat.no. 5-1608-000
(+ competent E. coli TOP10 cells, 20 rxns)
StarGate® Transfer Reagent Set Cat.no. 5-1603-001
2 Acceptor Vectors according to PPI Method
(Specifications of the Acceptor Vectors see below)

For PPI analysis we recommend to start with Acceptor Vectors equipped with the following features included in the following Kits:

Twin-Strep Cloning Kits	Vectors with Twin-Strep-tag® for N- and C-terminal fusion (available for E. coli and mammalia)
One-TAP Cloning Kits	Vectors with Twin-Strep-tag® for N- and C-terminal fusion (available for E. coli and mammalia)
Two-TAP Cloning Kits	Vectors with Twin-Strep / FLAG®-tag double tag for N- and C-terminal fusion (available for E. coli and mammalia)
SPINE Cloning Kit	Vectors with Strep-tag®II at N- and C-terminus (available for E. coli)

The recommended Acceptor Vectors are included in the PPI kits but can also be purchased separately (see technical information).

Overview of PPI Kits and single products

For further information on the PPI technology see protein interaction.

PPI Acceptor Vectors (included in the Kits)

Acceptor vectors included in the kits for protein protein interaction analysis

Overview of PPI Kits and single products

IBA's protein protein interaction analysis portfolio

StarPrimer D´Signer

Innovative software for StarGate® Entry Cloning Primer

Make use of the easy-to-use software, the StarPrimer D´Signer 3.0.0.3, to facilitate the design of

primers for the ENTRY cloning step to create the Donor Vector.