Acceptor vectors (E. coli)

pASG-IBA vectors

  • High-level expression in E. coli, also for toxic proteins
  • Tightly regulated expression due to anhydrotetracycline (AHT) inducible tetA promoter/operator
  • Option for periplasmic expression due to ompA signal sequence
  • No catabolite repression - no influence of medium components
  • Not influenced by the genetic background – wide choice of E. coli expression strains
  • Inexpensive induction with AHT.
 
pPSG-IBA vectors

StarGate¢ç Acceptor Vector Overview Table

 

Available Sequencing primers for E. coli Acceptor Vectors

Description Amount Cat. no.
Forward sequencing primer for pASG-IBA
and pASK-IBA vectors; HPLC-purified
1 nmol 5-0000-101
Reverse sequencing primer for pASG-IBA
and pASK-IBA vectors; HPLC-purified
1 nmol 5-0000-102
Forw. and rev. seq. primers for pASG-IBA
and pASK-IBA vectors; HPLC-purified
1 nmol
each
5-0000-104
Forward sequencing primer for pPSG-IBA
and pPR-IBA vectors; HPLC-purified
1 nmol 5-0000-111
Reverse sequencing primer for pPSG-IBA
and pPR-IBA vectors; HPLC-purified
1 nmol 5-0000-112
Forw. and rev. seq. primers for pPSG-IBA
and pPR-IBA vectors; HPLC-purified
1 nmol
each
5-0000-114
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