MyFi¢â is a newly developed, antibody-mediated, hot-start enzyme with unique properties, that offers 3.5x higher fidelity than native Taq and enhanced sensitivity and specificity, making MyFi a superior choice for cloning.
Features & Benefits• ³ôÀº Á¤È®¼º(fidelity), ³ôÀº ƯÀ̼º, °í°¨µµ
• »õ·Î¿î antibody±â¹ÝÀÇ hot-start polymerase • 10 kb±îÁö ÁõÆø °¡´É • »õ·ÎÀÌ °³¹ßµÈ buffer system • All-in-one master mix µµ ÀÖÀ½ • TA cloning °¡´É ('A' Overhang) Applications• ³ôÀº ƯÀ̼º°ú °í¼º´ÉÀ» ÇÊ¿ä·Î ÇÏ´Â PCR• TA cloning • Site-directed mutagenesis • Low copy PCR assays DescriptionMyFi¢â´Â antibody ¸¦ ±â¹ÝÀ¸·Î ÇÑ hot-start enzymeÀ¸·Î native Taq°ú ºñ±³ÇßÀ» ¶§ ¾à 3.5¹èÁ¤µµ fidelity°¡ ³ôÀº µ¶Æ¯ÇÑ ¼º´ÉÀ» °¡Áö°í ÀÖ½À´Ï´Ù. ÀÌ polymerase´Â MyFi buffer¿Í ÇÔ²² Á¦°øÀÌ µÇ´Âµ¥ buffer Á¶¼º¿¡´Â ultra-pureÇÑ dNTPs, MgCl2, enhancers°¡ ÃÖÀûÀÇ ³óµµ·Î ¼¯¿© ÀÖ¾î MyFi¢â ¼º´ÉÀ» ÇÑÃþ Çâ»ó½ÃÄÑÁÝ´Ï´Ù. MyFi¢â´Â cDNA libraries¸¦ ÁõÆøÇϰųª, ³ôÀº fidelity¸¦ ¿äÇÏ´Â low copy target, ³ôÀº GC-content¸¦ °¡Áø target µî ÃÖ°í 10kb ±æÀ̱îÁöÀÇ ÇÕ¼ºÀÌ ±î´Ù·Î¿î target¿¡ »ç¿ëÇϱâ ÀûÇÕÇÕ´Ï´Ù. ¶ÇÇÑ »ó¿Â¿¡¼ reaction mix¸¦ ¸¸µé ¼ö ÀÖ´Â Æí¸®ÇÔÀ» °¡Áö°í ÀÖ¾î ºñƯÀÌÀûÀÎ ÁõÆø°ú primer-dimer Çü¼ºÀ» ÇÇÇÒ ¼öÀÖ½À´Ï´Ù. MyFi¢â ¿ª½Ã MyFi¢â Mix¶ó´Â enhancers ¿Í stabilizer°¡ ÀÌ¹Ì µé¾îÀÖ´Â ready-to-use 2X mix ŸÀÔÀÌ ÀÖ¾î trouble-freeÇÑ PCR set-up¿¡ ¿ëÀÌÇÕ´Ï´Ù. Single tube·Î Á¦°øµÇ±â ¶§¹®¿¡ pipetting stepsÀ» ÁÙ ÀÏ ¼ö ÀÖ¾î pipetting error¸¦ ÁÙÀÌ°í ¿À¿° ¹æÁö¸¦ Çϴµ¿½Ã¿¡ Æí¸®ÇÏ°Ô ½ÇÇèÀÌ °¡´ÉÇÕ´Ï´Ù. NotesMyFi is a Trademark of Bioline.
Components
VolumeBIO-21117: 125 x 25¥ìl Reactions: 1 x 125¥ìl
BIO-21118: 250 x 25¥ìl Reactions: 1 x 250¥ìl
BIO-21119: 1250 x 25¥ìl Reactions: 2 x 625¥ìl Concentration2u/¥ìl Storage & StabilityAll components should be stored at -20¡ÆC upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label. Shipping conditionsOn Dry Ice or Blue Ice. Fact sheet [MyFi¢â_DNA_Polymerase_product_sheet.pdf] (407,510kb) protocol [MyFi¢â_DNA_Polymerase_protocol.pdf] (138,161kb) |
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