MyFi¢â Mix is a ready-to-use 2x mix, specifically designed for high-fidelity amplification of up to 10kb of genomic DNA.
Owing to its antibody-based hot-start property, MyFi Mix has the added convenience of room temperature reaction assembly, avoiding unwanted non-specific amplification and primer-dimer formation. Features & Benefits• ³ôÀº Á¤È®¼º(fidelity), ³ôÀº ƯÀ̼º, °í°¨µµ
• »õ·Î¿î antibody±â¹ÝÀÇ hot-start polymerase • 10 kb±îÁö ÁõÆø °¡´É • »õ·ÎÀÌ °³¹ßµÈ buffer system • All-in-one master mix µµ ÀÖÀ½ • TA cloning °¡´É ('A' Overhang) Applications• ³ôÀº ƯÀ̼º°ú °í¼º´ÉÀ» ÇÊ¿ä·Î ÇÏ´Â PCR
• TA cloning • Site-directed mutagenesis • Low copy PCR assays DescriptionMyFi¢â Mix´Â antibody ¸¦ ±â¹ÝÀ¸·Î ÇÑ hot-start enzymeÀ¸·Î native Taq°ú ºñ±³ÇßÀ» ¶§ ¾à 3.5¹èÁ¤µµ fidelity°¡ ³ôÀº µ¶Æ¯ÇÑ ¼º´ÉÀ» °¡Áö°í ÀÖ½À´Ï´Ù. Buffer¿Í enzymeÀÌ ¼¯¿© ÀÖ´Â ÀÌ Á¶¼º¿¡´Â ultra-pureÇÑ dNTPs, MgCl2, enhancers°¡ ÃÖÀûÀÇ ³óµµ·Î ¼¯¿© ÀÖ¾î enzymeÀÇ ¼º´ÉÀ» ÇÑÃþ Çâ»ó½ÃÄÑÁÝ´Ï´Ù.
MyFi¢â Mix´Â cDNA libraries¸¦ ÁõÆøÇϰųª, ³ôÀº fidelity¸¦ ¿äÇÏ´Â low copy target, ³ôÀº GC-content¸¦ °¡Áø target µî ÃÖ°í 10kb ±æÀ̱îÁöÀÇ ÇÕ¼ºÀÌ ±î´Ù·Î¿î target¿¡ »ç¿ëÇϱâ ÀûÇÕÇÕ´Ï´Ù. ¶ÇÇÑ »ó¿Â¿¡ ³ëÃâµÇ¾îµµ Å« ¿µÇâÀ» ¹ÞÁö ¾Ê±â ¶§¹®¿¡ ¿Âµµ¿¡ ºñ±³Àû ¹Î°¨ÇÑ ´Ù¸¥ Taq enzyme¿¡ ºñÇØ ºñƯÀÌÀûÀÎ ÁõÆø°ú primer-dimer Çü¼ºÀ» ÇÇÇÒ ¼öÀÖ½À´Ï´Ù. MyFi¢â Mix´Â trouble-freeÇÑ PCR set-up¿¡ ¿ëÀÌÇÕ´Ï´Ù. Single tube·Î Á¦°øµÇ±â ¶§¹®¿¡ pipetting stepsÀ» ÁÙ ÀÏ ¼ö ÀÖ¾î pipetting error¸¦ ÁÙÀÌ°í ¿À¿° ¹æÁö¸¦ ÇÏ´Â µ¿½Ã¿¡ Æí¸®ÇÏ°Ô ½ÇÇèÀÌ °¡´ÉÇÕ´Ï´Ù. NotesMyFi is a Trademark of Bioline.
Components
VolumeBIO-25049: 100 x 50¥ìl Reactions: 2 x 1.25ml
BIO-25050: 500 x 50¥ìl Reactions: 10 x 1.25ml
Concentration2xStorage & StabilityAll components should be stored at -20¡ÆC upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity is retained until the expiry date indicated on the outer box label.
Shipping conditionsShipped on Dry Ice or Blue Ice. protocol [pi_50145_myfi_mix_v2.pdf] (429,535kb) Fact sheet [MyFi¢â_DNA_Polymerase_product_sheet.pdf] (407,510kb) |
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