MyTaq¢â HS Mix
CAT# PRODUCT SIZE STORAGE ¼ÒºñÀÚ°¡ ÁÖ¹®
BIO-25045MyTaq HS Mix, 2x200 RXNSat -20¡ÆC-·Î±×ÀÎ
BIO-25046MyTaq HS Mix, 2x1000 RXNSat -20¡ÆC-·Î±×ÀÎ
-·Î±×ÀÎ
mytaqhs_2.jpgMyTaq¢â HS Mix is a ready-to-use 2x mix for fast, highly-specific, hot-start PCR.
MyTaq HS Mix is powered by antibody mediated hot-start and does not exhibit polymerase activity during room-temperature reaction set-up, thus reducing non-specific amplification.
 
 
 

 

Features & Benefits

 
• »õ·Ó°Ô °³¹ßµÈ antibody ±â¹ÝÀÇ hot-start polymerase
• ³ôÀº specificity ¿Í ¿ì¼öÇÑ performance
• »õ·Î¿î buffer : ultra-pure dNTPs ¿Í MgCl2
• ¹Ù·Î gel loadingÀÌ °¡´ÉÇÏ°Ô red-dye Æ÷ÇÔ °¡´É
• °£ÆíÇÑ all-in-one mastermix

Applications

High-throughput PCR Assays with prolonged reaction setup on the bench or liquid handling 
Amplification of challenging targets susceptible to mispriming 
Multiplexing
Colony PCR
Specific amplification of difficult templates (GC rich)
Genotyping
TA cloning
Two-Step RT-PCR

Description

MyTaq¢â HS Mix´Â Complex genomic DNA¿Í °° Àº ¾î·Á¿î template¸¦ º¸´Ù ½±°í Á¤È®ÇÏ°Ô ÁõÆøÇϱâ À§ÇØ Bioline¿¡¼­ »õ·Ó°Ô °³¹ßÇÑ antibody ±â¹ÝÀÇ hot start PCR enzyme ÀÔ´Ï´Ù. ¼öÀ², °¨µµ, ¼Óµµ¸¦ ÁÁ°Ô ÇÒ ¼ö ÀÖµµ·Ï ÃֽŠ±â¼úÀ» ÀÌ¿ëÇÏ¿© PCR enzyme°ú DNA¿ÍÀÇ Ä£È­·ÂÀ» ³ô¿©ÁÖ¾ú½À´Ï´Ù. »ó¿Â¿¡¼­ reaction mix¸¦ ¸¸µé ¼ö ÀÖÀ¸¹Ç·Î ºñƯÀÌÀûÀÎ ÁõÆø°ú primer-dimer Çü¼ºÀ» ÇÇÇÒ ¼ö ÀÖ½À´Ï´Ù. ¶ÇÇÑ ÀÌ mastermix¿¡´Â dNTPs, MgCl2, enhancerµéÀÌ ÃÖÀûÀÇ ³óµµ·Î µé¾î°¡ À־ optimization ¾øÀ̵µ ¿ì¼öÇÑ °á°ú¸¦ ¸¸µé ¼ö ÀÖ½À´Ï´Ù. ÀÌ¹Ì ¸ðµç ÇÊ¿ä½Ã¾àÀÌ ¼¯ÀÎ MyTaq¢â HS Mix´Â ready-to-use 2x mix typeÀ¸·Î ºü¸¥ °á°ú¸¦ °¡Á®¿Ã ¼ö ÀÖ´Â °í°¨µµÀÇ PCR enzymeÀÔ´Ï´Ù. ÀÌ Á¦Ç°Àº ºü¸¥ cycling conditionÀ¸·Î ÁøÇà ÇÒ ¼ö ÀÖµµ·Ï setting µÇ¾î °¨µµ³ª ¼öÀ²Àº ³ôÀ¸¸é¼­ ½ÇÇè ½Ã°£Àº ´ÜÃà ½Ãų ¼ö ÀÖ½À´Ï´Ù.
 

Notes

MyTaq is a trademark of Bioline.

Concentration

2x

Storage & Stability

All components are shipped on dry/blue ice and should be stored at -20¡ÆC upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.
Components may also be stored at +4¡ÆC if required, although storage at -20¡ÆC is recommended. When stored at +4¡ÆC, reagents will remain stable for a period of 2 weeks from date of receipt.

protocol [pi_50152_mytaq_hs_mix_v2.pdf] (437,002kb)
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