IDT
IDT
IBAfect principle 1) IBAfect (cationic lipids) condenses DNA to compact structures within DNA/RNA/lipid-complexes.
2) These positively charged DNA/RNA/lipid-complexes are binding to and are entering the cell by endocytosis.
3) The DNA/RNA/lipid-complex acts as a "proton sponge" that buffers the endosomal pH, since the hydrophilic unit contains several sites which can be protonated.
4) Continuous proton influx induces osmotic swelling and rupture. Nucleic acids are released simultaneously from the DNA/RNA/lipid-complex by Progressive Proton-assisted Lipid Layer Disintegration (P.P.L.L.D.).
5) Nucleic acids are released into the cytosol. |
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