RANGER DNA Polymerase is a hot-start enzyme, possessing 5¢¥-3¢¥ DNA polymerase and 3¢¥-5¢¥ proofreading exonuclease activities, thus offering both high-fidelity and enhanced specificity.
Features & Benefits• ºü¸¥ ¼ÓµµÀÇ antibody±â¹Ý hot-start
• Ultra-pureÇÑ dNTPs¿Í MgCl2¸¦ Æ÷ÇÔÇÑ »õ·Î¿î buffer system • Taq º¸´Ù ³ôÀº Á¤È®µµ(fidelity) • º¸´Ù ½¬¿î all-in-one master mix ŸÀÔµµ °¡´É Applications• ÃÖ°í 25 kbÀÇ human genomic DNA ÁõÆø¿¡µµ ÀûÇÕ
• TA cloning °¡´É DescriptionRANGER DNA polymerase´Â hot-start enzymeÀ¸·Î 5'¡æ3'°ú 3'¡æ5' exonuclease È°¼ºÀ» °¡Áö°í ÀÖ¾î high-fidelity¿Í °í°¨µµ Ư¼ºÀ» ÀÚ¶ûÇÕ´Ï´Ù. RANGERÀÇ È¿°ú¸¦ ±Ø´ëÈÇϱâ À§ÇØ »õ·Ó°Ô °³¹ßµÈ buffer systemµµ ÇÔ²² Á¦°øÇÕ´Ï´Ù. ¸Å¿ì ¿¹¹ÎÇÑ targetÀ̳ª 10 kb ÀÌ»óÀÇ targetÀ» Àü¹®ÀûÀ¸·Î ÁõÆøÇϱâ À§ÇØ easy-to-use ŸÀÔÀ¸·Î ¸¸µé¾î Á³½À´Ï´Ù. RANGER ¿ª½Ã antibody ±â¹ÝÀÇ hot start ±â´ÉÀÌ Àֱ⠶§¹®¿¡ »ó¿Â¿¡¼ reaction sampleÀ» ¸¸µé ¼ö ÀÖ´Â Æí¸®ÇÔÀ» °¡Áö°í ÀÖ¾î ºñƯÀÌÀûÀÎ ÁõÆø°ú primer-dimer Çü¼ºÀ» ÇÇÇÒ ¼ö ÀÖ½À´Ï´Ù. ÀÌ enzyme¿¡´Â ±¸¼ºÇ°À¸·Î 5X RANGER reaction buffer°¡ ÇÔ²² µé¾îÀִµ¥ ¿©±â¿¡ µé¾îÀÖ´Â ultra-pure dNTPs, MgCl2, enhancersµîÀÌ ÃÖÀûÀÇ ³óµµ·Î ¼¯¿© ÀÖ¾î¼ ÁõÆøÀ» º¸´Ù È¿°úÀûÀ¸·Î ÇÒ ¼ö ÀÖ°Ô µµ¿ÍÁÝ´Ï´Ù.
RANGER´Â sequencingÀ̳ª chromosomal translocation breakpointsÀÇ mapping, structural variations¿Í °°ÀÌ ±ä targetÀ» PCR ÇÒ ¶§ ¸Å¿ì À¯¿ëÇÑ Á¦Ç°ÀÔ´Ï´Ù.RANGER¿¡µµ ready-to-use typeÀÇ 2X mix°¡ ÀÖ½À´Ï´Ù. Reaction set up ´Ü°è¿¡¼´Â polymerase°¡ ºñÈ°¼º »óÅÂÀ̱⠶§¹®¿¡ non-specificÇÑ ÁõÆøÀ» ÁÙÀÏ ¼ö ÀÖ½À´Ï´Ù. RANGER MixÀÇ Æ¯º°ÇÑ Á¶ÇÕ ¶§¹®¿¡ ¸Å¿ì °í°¨µµ »óÅ·ΠÁõÆøÀÌ °¡´ÉÇÏ°í fidelity ¿ª½Ã ³ô½À´Ï´Ù. ÀÌ Á¦Ç°Àº trouble-free PCR reaction set up¿¡ ÇÊ¿äÇÑ ¸ðµç reagent¸¦ Æ÷ÇÔÇÏ´Â m astermix ŸÀÔÀ¸·Î ½ÇÇèÀÚÀÇ Æí¸®ÇÔÀ» ³ôÀÌ°íÀÚ ÇϳªÀÇ tube¿¡ ´ã°Ü ÀÖ½À´Ï´Ù. ÀÌ´Â pipetting stepÀ» ÁÙÀÌ°í È¿À²¼º°ú »ý»ê¼ºÀ» ³ôÀ̴µ¥ µµ¿òÀÌ µË´Ï´Ù. NotesPurchase of this product does not convey a licence to perform any patented process. Components
VolumeBIO-21121: 62 x 50¥ìl Reactions: 1 x 62.50¥ìlBIO-21122: 125 x 50¥ìl Reactions: 1 x 125¥ìl
BIO-21123: 625 x 50¥ìl Reactions: 2 x 312.50¥ìl Concentration4u/¥ìlStorage & StabilityAll components should be stored at -20¡ÆC upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.
RANGER DNA Polymerase may also be stored at +4¡ÆC for up to 2 weeks, although storage at -20¡ÆC is recommended. Shipping conditionsOn Dry Ice or Blue Ice. Fact sheet [RANGER_DNA_Polymerase_product_sheet.pdf] (205,520kb) protocol [RANGER_DNA_Polymerase_protocol.pdf] (138,491kb) |
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