RANGER Mix
CAT# PRODUCT SIZE STORAGE ¼ÒºñÀÚ°¡ ÁÖ¹®
BIO-25052500 Reactions, 2x RANGER Mix500 RXNSat -20¡ÆC-·Î±×ÀÎ
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RANGER Mix is a ready-to-use 2x mix, designed for the amplification of templates of 10kb or greater.
The advanced formulation of RANGER Mix enables extreme sensitivity and increased fidelity. For convenience, all of the components necessary for trouble-free PCR reactions are supplied in one tube to reduce the number of pipetting steps and improve efficiency and reproducibility.
 
 
 
 

Features & Benefits

• ºü¸¥ ¼ÓµµÀÇ antibody±â¹Ý hot-start
• Ultra-pureÇÑ dNTPs¿Í MgCl2¸¦ Æ÷ÇÔÇÑ »õ·Î¿î buffer system
• Taq º¸´Ù ³ôÀº Á¤È®µµ(fidelity)
• º¸´Ù ½¬¿î all-in-one master mix ŸÀÔµµ °¡´É

Applications

• ÃÖ°í 25 kbÀÇ human genomic DNA ÁõÆø¿¡µµ ÀûÇÕ
• TA cloning °¡´É

Description

RANGER DNA polymerase´Â hot-start enzymeÀ¸·Î 5'¡æ3'°ú 3'¡æ5' exonuclease È°¼ºÀ» °¡Áö°í ÀÖ¾î high-fidelity¿Í °í°¨µµ Ư¼ºÀ» ÀÚ¶ûÇÕ´Ï´Ù. RANGERÀÇ È¿°ú¸¦ ±Ø´ëÈ­Çϱâ À§ÇØ »õ·Ó°Ô °³¹ßµÈ buffer systemµµ ÇÔ²² Á¦°øÇÕ´Ï´Ù. ¸Å¿ì ¿¹¹ÎÇÑ targetÀ̳ª 10 kb ÀÌ»óÀÇ targetÀ» Àü¹®ÀûÀ¸·Î ÁõÆøÇϱâ À§ÇØ easy-to-use ŸÀÔÀ¸·Î ¸¸µé¾î Á³½À´Ï´Ù. RANGER ¿ª½Ã antibody ±â¹ÝÀÇ hot start ±â´ÉÀÌ Àֱ⠶§¹®¿¡ »ó¿Â¿¡¼­ reaction sampleÀ» ¸¸µé ¼ö ÀÖ´Â Æí¸®ÇÔÀ» °¡Áö°í ÀÖ¾î ºñƯÀÌÀûÀÎ ÁõÆø°ú primer-dimer Çü¼ºÀ» ÇÇÇÒ ¼ö ÀÖ½À´Ï´Ù. ÀÌ enzyme¿¡´Â ±¸¼ºÇ°À¸·Î 5X RANGER reaction buffer°¡ ÇÔ²² µé¾îÀִµ¥ ¿©±â¿¡ µé¾îÀÖ´Â ultra-pure dNTPs, MgCl2, enhancersµîÀÌ ÃÖÀûÀÇ ³óµµ·Î ¼¯¿© À־ ÁõÆøÀ» º¸´Ù È¿°úÀûÀ¸·Î ÇÒ ¼ö ÀÖ°Ô µµ¿ÍÁÝ´Ï´Ù.
RANGER´Â sequencingÀ̳ª chromosomal translocation breakpointsÀÇ mapping, structural variations¿Í °°ÀÌ ±ä targetÀ» PCR ÇÒ ¶§ ¸Å¿ì À¯¿ëÇÑ Á¦Ç°ÀÔ´Ï´Ù.RANGER¿¡µµ ready-to-use typeÀÇ 2X mix°¡ ÀÖ½À´Ï´Ù. Reaction set up ´Ü°è¿¡¼­´Â polymerase°¡ ºñÈ°¼º »óÅÂÀ̱⠶§¹®¿¡ non-specificÇÑ ÁõÆøÀ» ÁÙÀÏ ¼ö ÀÖ½À´Ï´Ù. RANGER MixÀÇ Æ¯º°ÇÑ Á¶ÇÕ ¶§¹®¿¡ ¸Å¿ì °í°¨µµ »óÅ·ΠÁõÆøÀÌ °¡´ÉÇÏ°í fidelity ¿ª½Ã ³ô½À´Ï´Ù. ÀÌ Á¦Ç°Àº trouble-free PCR reaction set up¿¡ ÇÊ¿äÇÑ ¸ðµç reagent¸¦ Æ÷ÇÔÇÏ´Â m astermix ŸÀÔÀ¸·Î ½ÇÇèÀÚÀÇ Æí¸®ÇÔÀ» ³ôÀÌ°íÀÚ ÇϳªÀÇ tube¿¡ ´ã°Ü ÀÖ½À´Ï´Ù. ÀÌ´Â pipetting stepÀ» ÁÙÀÌ°í È¿À²¼º°ú »ý»ê¼ºÀ» ³ôÀ̴µ¥ µµ¿òÀÌ µË´Ï´Ù.

Notes

Purchase of this product does not convey a licence to perform any patented process.

Components

Reagent
100 Reactions
500 Reactions
RANGER Mix, 2x
2x 1.25ml
10 x 1.25ml

Volume

BIO-25051: 100 x 50¥ìl Reactions: 2 x 1.25ml 
BIO-25052: 500 x 50¥ìl Reactions: 10 x 1.25ml

Concentration

2x

Storage & Stability

All components should be stored at -20¡ÆC upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.
Components may also be stored at +4¡ÆC if required, although storage at -20¡ÆC is recommended. When stored at +4¡ÆC, reagents will remain stable for a period of 2 weeks from date of receipt.

Shipping conditions

Shipped on Dry Ice or Blue Ice
protocol [ranger_mix_manual.pdf] (147,007kb)
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