Strep-Tactin® Superflow® & Strep-Tactin® Superflow® high capacity

for purification of Strep-tag® II proteins by gravity flow, low pressure or FPLC

Both Strep-Tactin® Superflow® resins

combine outstanding flow characteristics with superior mechanical stability
are suitable for increased flow rates and useful for purification of large protein complexes
come in pre-packed Strep-Tactin® columns for gravity flow applications
or in the Strep-Tactin® cartridges H-PR* ("highly pressure-resistant") for work with automated workstations
yield much higher protein purity (> 95 %) than 6xHistidine-tag
purification on Ni-NTA Superflow®

While Strep-Tactin® Superflow® is available at very competitive prices the Strep-Tactin® Superflow® high capacity resin offers improved binding capacity but identical high protein purity compared to standard Strep-Tactin® Superflow®. Thus, it is the perfect solution for Strep-tag® protein purification.

The Strep-Tactin® Superflow® high capacity resin

provides 2 to 3 times higher binding capacity than standard Strep-Tactin® Superflow® (150 – 500 nmol or 3 – 15 mg recombinant Strep-tag® fusion protein per ml sedimented resin)
enables a cost effective purification of higher protein amounts with optimal purity
isolation of comparable protein yields with His-tag/Ni-NTA purification

Get more protein with optimal purity!

Comparison of Strep-Tactin Superflow with Ni-NTA Superflow in respect to binding capacity and protein purity

Comparison of Strep-Tactin® Superflow® vs. Superflow® high capacity

Purification of Strep-tag II proteins via Strep-Tactin Superflow

Special Application

Purification of intact virus like particle using Strep-Tactin Supeflow

Figure 1, left to right: M; Rainbow Marker RPN 755,
GE Healthcare Biosciences Corp.,
1 and 2; different elution fractions of VLPs
fused to Strep-tag II after one-step Strep-Tactin
affinity purification from a crude lysate of E. coli.

Strep-Tactin® Superflow® for intact virus like particle (VLP) 4.8 MDa purification

1 liter culture was induced at an OD₆₀₀ of 0.6 using anhydrotetracycline (AHT) and protein expression was performed at 37 °C for 3 hours at 200 rpm. Then, cells were pelleted, resuspended in 20 ml Buffer W (100 mM Tris-Cl, 150 mM NaCl, 1 mM EDTA) and sonicated. The insoluble material was pelleted and the crude lysate was loaded on a Strep-Tactin® Superflow® column at a flow rate of 1 ml/minute. After washing off the host proteins, the VLPs fused to Strep-tag® II were eluted using 2.5 mM desthiobiotin in Buffer W. pASK-IBA7 was used for cloning.

For the purification of intact VLPs the careful choice of both an ideal affinity tag system and the resin support is important:

1) The affinity chromatography process should be mild in order to keep the VLPs intact which is met by using Strep-tag®.

2) The resin support packing within the column must allow penetration of the large VLPs.

While MacroPrep® carriers did not meet criterion (2), since the viral particles accumulated on top of the column, Sepharose® and Superflow® allowed the purification. Superflow® can be used in FPLC systems whereas Sepharose® is only suitable for gravity flow purification.

These results were kindly provided by L. Stöckl and B. Brandenburg, Robert-Koch- Institute, Berlin.

Brandenburg B., Stockl L., Gutzeit C., Roos M., Lupberger J., Schwartlander R., Gelderblom H., Sauer I. M., Hofschneider P. H. and Hildt E. (2005)

A Novel System for Efficient Gene Transfer Into Primary Human Hepatocytes Via Cell-Permeable Hepatitis B Virus–like Particle

HEPATOLOGY (42),1300-1309

Specifications of Strep-Tactin® Superflow resin

	Strep-Tactin® Superflow®	Strep-Tactin® Superflow® high capacity
Binding capacity	1 ml sedimented resin (corresponding to 2 ml of a 50 % suspension) can be used for the one-step purification of 50 to 100 nmol (up to 3 mg) recombinant protein	1 ml sedimented resin (corresponding to 2 ml of a 50 % suspension) can be used for the one-step purification of 150 to 500 nmol or 3 -15 mg recombinant protein
Support	Superflow 6 (6 % agarose, crosslinked)	Superflow 6 (6 % agarose, crosslinked)
Bead size	60-160 µm, spherical	60-160 µm, spherical
Linear flow rate	100-300 cm/h is recommended for Strep-tag® purification	100-300 cm/h is recommended for Strep-tag® purification
Exclusion limit	6x10⁶Da	6x10⁶Da
Form (bulk)	50 % suspension in 100 mM Tris/HCl pH 8.0, 150 mM NaCl, 1 mM EDTA	50 % suspension in 100 mM Tris/HCl pH 8.0, 150 mM NaCl, 1 mM EDTA
Stability	at least 6 months after shipment	at least 6 months after shipment
Storage	4 °C, DO NOT FREEZE	4 °C, DO NOT FREEZE
Shipment	RT	RT

For a complete overview of the Strep-Tactin® resin specifications, please click here.

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