MyTaq Extract-PCR Kit provides quick and easy extraction and amplification of DNA from a variety of tissue types. The MyTaq Extract-PCR Kit maximizes sensitivity while simultaneously minimizing contamination risks to deliver greater experiment success rates.
Features & Benefits• ºü¸¥ extraction protocol - 15ºÐ ³»¿¡ PCR-ready DNAÀ» ³ôÀº È¿À²·Î ÃßÃâ
• º¹ÀâÇÑ DNA ÃßÃâ °úÁ¤À» ´ëüÇÒ ¼ö ÀÖÀ½ • Mammalian tissues¿¡¼ ´ë¿ë·® genotyping½Ã ¸Å¿ì ÀûÇÕ • Æí¸®ÇÑ single-tube ¹ÝÀÀÀ¸·Î ¿À¿°À» ÃÖ¼ÒÈ ÇÔ • MyTaq¢âHS Mix´Â ºü¸£°í ³ôÀº ƯÀ̼º ÁõÆø¿¡ ÀûÇÕ Applications• High-throughput genotyping from mammalian tissues• Transgenes¸¦ detection • Knockout analysis Instrument CompatibilityCompatible with all standard PCR instruments.DescriptionMyTaq¢â Extract-PCR Kit´Â ¿©·¯ °¡ÁöÀÇ tissue Á¾·ù·ÎºÎÅÍ DNA¸¦ ÃßÃâÇÏ°í ÁõÆøÇÏ´Â °úÁ¤À» º¸´Ù ½±°í ºü¸£°Ô Á¦°øÇϱâ À§ÇØ
¸¸µé¾îÁ³½À´Ï´Ù. ¿©·¯ ´Ù¸¥ DNA extraction ¹æ¹ýÀº ½Ã°£ÀÌ ¿À·¡°É¸®°í, stepÀÌ ±æ°Å³ª phenolÀ» ÀÌ¿ëÇÏ¿© ÃßÃâÇÏ°í, overnight À¸·Î incubationÀ» ÇÏ´Â µî ¸¹Àº ºÒÆíÇÔÀÌ µû¸¨´Ï´Ù. MyTaq¢â Extract-PCR kit´Â Mouse tailÀ̳ª earµî solid tissue ¿¡¼ºÎÅÍ ´Ù¸¥ ¿©·¯ tissue type¿¡ À̸£±â±îÁö ¿©·¯ sampleµé·ÎºÎÅÍ DNA¸¦ ÃßÃâÇÏ°í À̸¦ ÀÌ¿ëÇÏ¿© ÁõÆøÇÏ´Â ½ÇÇèÀ» º¸´Ù Æí¸®ÇÏ°Ô Á¦°øÇÕ´Ï´Ù. DNA ÃßÃâ °úÁ¤Àº ÇϳªÀÇ tube¿¡¼ ÀÌ·ç¾îÁö°í ¿©·¯ ¹øÀÇ washing stepÀÌ ÇÊ¿äÇÏÁö ¾Ê¾Æ sample loss³ª ¿À¿°ÀÇ À§Çè¿¡¼ ¾ÈÀüÇÕ´Ï´Ù. ÃßÃâµÈ DNA´Â kit¿¡ Æ÷ÇÔµÈ antibody ±â¹ÝÀÇ hot start ±â´ÉÀ» °¡Áø MyTaq¢â HS Red Mix¸¦ »ç¿ëÇÏ¿© ÁõÆøÀÌ °¡´ÉÇÕ´Ï´Ù. ÀÌ MyTaq¢â HS Mix´Â fast cycling conditionÀ¸·Î »ç¿ëÀÌ °¡´ÉÇÏ¿© reaction timeÀ» ÁÙÀÌ¸é¼ PCRÀÇ °¨µµ³ª ¼öÀ²À» ³ôÀÏ ¼ö ÀÖ½À´Ï´Ù. Single tube lysis protocol°ú MyTaq¢â HS Red Mix¸¦ ÇÔ²² »ç¿ëÇÔÀ¸·Î½á ¿À¿°ÀÇ À§ÇèÀº ÇöÀúÈ÷ ÁÙÀÌ°í °¨µµ´Â ÃÖ°í·Î ³ôÀÏ ¼ö°¡ ÀÖÀ¸¸ç ½ÇÇè½Ã°£ ¿ª½Ã ´«¿¡ ¶ç°Ô ÁÙ¾îµé°Ô µË´Ï´Ù. »õ·Ó°Ô °³¹ßµÈ MyTaq¢â HS Red Mix´Â PCR reaction ÀÚüÀÇ È°¼º¿¡´Â ¿µÇâÀ» ÁÖÁö ¾ÊÀ¸¸é¼ ¹Ù·Î gel loadingÀÌ °¡´ÉÇÏ°Ô red dye°¡ Æ÷ÇÔÀÌ µÇ¾îÀֱ⠶§¹®¿¡ ÀÌ ¶ÇÇÑ Bioline Á¦Ç°¸¸ÀÌ °¡Áø ÀåÁ¡À̶ó°í º¼ ¼ö ÀÖ½À´Ï´Ù. NotesMyTaq is a trademark of Bioline. Components
Concentration2x QC specificationsBioline operates under ISO 9001 Management System. MyTaq Extract-PCR Kit and its components are extensively tested for activity, processivity, efficiency, heat activation, sensitivity, absence of nuclease contamination and absence of nucleic acid contamination prior to release. Storage & StabilityAll components should be stored at -20¡ÆC upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label. Shipping conditionsShipped on Blue Ice.Fact sheet [mytaq_extract_pcr_kit_fact_sheet.pdf] (510,774kb) protocol [pi_50150_mytaq_extract_pcr_kit_v3.pdf] (343,278kb) |
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