IDT
IDT
Strep-Tactin® MacroPrep®The MacroPrep® resin exhibits non-specific binding properties differing from those of Sepharose® or Superflow® and can, thus, be recommended in cases where suboptimal results are obtained with Sepharose® or Superflow® and vice versa (see applications).
MacroPrep® is available in a multitude of formats, click here for all available column formats.
H-PR cartridges For purification of Strep-tag® II proteins by gravity flow or under low pressureStrep-Tactin® MacroPrep® is available as pre-packed gravity flow columns or as H-PR cartridges (for chromatography workstations with 10-32 connections).
 Strep-Tactin® Spin columns - for rapid parallel purification of Strep-tag® proteins
 Strep-Well - the perfect solution for automated, high-throughput purification of Strep-tag® proteins
Strep-Well HT 50 Purification Plates provide purification of up to 200 µg recombinant protein per well of 96 samples in parallel.
These 96-well affinity chromatography modules are the perfect solution for rapid or high-throughput purification of Strep-tag® proteins. The plates are pre-loaded with Strep-Tactin® and simply have to be re-hydrated and equilibrated before usage. See Strep-Well procedure under technical information. Gravity flow StrepMAB-Classic MacroPrep® ColumnAn antibody-based option for Strep-tag® protein purificationThis new antibody-based purification column with StrepMAB-Classic immobilized to MacroPrep® provides an alternative for purification of Strep-tag® proteins. Using this column as second step following purification via Strep-Tactin® protein purity can be increased to over 99% while only one tag – the Strep-tag® – is used.
In protein:protein interaction analysis e.g. this column is used in the so-called One-TAP system enabling a second, completely independent purification step with respect to resin (MacroPrep® vs Superflow®) and affinity receptor (mAB vs Strep-Tactin®). For some recombinant proteins, purification characteristics on Strep-Tactin® MarcoPrep® differ from those on Strep-Tactin® Sepharose®
Lane 1, molecular weight standard (kDa); Lane 2, Strep-Tactin® MacroPrep purified protein sample; Lane 3, Strep-Tactin® Sepharose® purified protein sample; Lane 4, soluble raw lysate as applied on each column The Coomassie stained SDS gel shows the purification of a recombinant protein which exhibits exceptionally high non-specific protein binding. In this case, we have been able to remove the contaminants nearly quantitatively using the Strep-Tactin® MacroPrep® resin while the contaminants co-eluted with the recombinant protein after the same purification protocol using the Strep-Tactin® Sepharose® resin. (Please note, that normally behaving recombinant proteins can be purified in a single step to homogeneity using Strep-Tactin® Sepharose®). This example shows that it may be reasonable to change the resin if non-specific protein binding occurs.
MacroPrep® is for example for plant proteins advantagous, where the purification with MacroPrep® leads to better protein yields Strep-Tactin Spin columnsStrep-Tactin® Spin Column Purification of three different Strep-tag® proteins.
lanes 1 and 8: marker; lanes 2, 4 and 6: bacterial crude lysate; lanes 3, 5 and 7: purified Strep-tag proteins Strep-Well purification example Strep-tag® vector constructs for the expression of GAPDH from S. aureus (37.5 kDa), GFP from A. victoria (28.1 kDa), human GSHH (23.5 kDa) and Azurin from P. aeruginosa (15.1 kDa) were transformed into E. coli and plated on selective medium. 96 colonies were randomly picked to inoculate 5 ml cultures. Protein expression was induced by addition of anhydrotetracycline or IPTG. Cells were harvested, lysed and Strep-tag® proteins were purified on a Strep-Tactin® HT Purification Plate. 8 µl of 20 elution fractions were loaded on a SDS-PAGE, gel was stained with Coomassie. Specifications of Strep-Tactin MacroPrep resin
Specifications of Gravity flow StrepMAB-Classic MacroPrep® Columns
|
사용후기 작성
|
