MagStrep: Strep-Tactin¢ç coated Magnetic Beads
MagStrep (Strep-Tactin¢ç coated Magnetic Beads)
- fast purification of Strep-tag¢ç proteins in batch format
- allowing protein purification in variable volumes even very small ones
- applicable in combination with standard buffers, Strep-tag¢ç II and One-Strep-tag fusion proteins
- now also available as high capacity (HC) format for high yield batch purification
Please note that for expression rates below 1 mg/liter culture, i.e. if the target protein concentration in the crude extract is less than 100 µg/ml, we highly recommend to use our Strep-Tactin¢ç purification columns or for high-throughput applications our Strep-Well HT Purification Plates instead of performing batch purification with MagStrep. In General One-STrEP-tag leads to better results in batch purification due to its higher affinity to Strep-Tactin¢ç. |
Available Formats:
MagStrep "type2" Beads and the MagStrep "type2HC" Beads with higher Strep-tag¢ç binding
capacity compared to MagStrep "type2". |
Specifications of MagStrep Beads
|
MagStrep "type 2" |
MagStrep "type 2HC" |
Bead size |
0.5-1.5 µm |
0.5-1.5 µm |
Magnetite content |
20 - 40 % |
20 - 40 % |
Binding capacity Strep-tag¢ç II fusion proteins: |
0.25 -0.5 nmol/mg beads |
0.5 -1.0 nmol/mg beads |
Form |
50 mg/ml suspension in Dulbecco¢¥s PBS; 0.1 % bovine serum albumin; 0.02 % NaN3 id. |
50 mg/ml suspension in Dulbecco¢¥s PBS; 0.1 % bovine serum albumin; 0.02 % NaN3 id. |
Stability |
12 months after shipment |
12 months after shipment |
Storage |
4 ¡ÆC, DO NOT FREEZE id |
4 ¡ÆC, DO NOT FREEZE id |
Shipment |
RT id. |
RT id. |
Magnetic Separator
for 24 reaction tubes (12x 1,5 ml and 12x 2 ml)
Experimental data: Protein purification using MagStrep "type2" and "type2 HC" Beads
Proteins were purified from crude E. coli extracts following standard protocol
Lane 1 : Marker
Lane 2-4: Strep-tag¢ç II proteins purified with MagStrep "type2"
Lane 5-7: Strep-tag¢ç II proteins purified with MagStrep "type2HC"