Strep-Tactin Resins (MagStrep)

MagStrep: Strep-Tactin¢ç coated Magnetic Beads

MagStrep (Strep-Tactin¢ç coated Magnetic Beads)
  • fast purification of Strep-tag¢ç proteins in batch format
  • allowing protein purification in variable volumes even very small ones
  • applicable in combination with standard buffers, Strep-tag¢ç II and One-Strep-tag fusion proteins
  • now also available as high capacity (HC) format for high yield batch purification
Please note that for expression rates below 1 mg/liter culture, i.e. if the target protein concentration in the crude extract is less than 100 µg/ml, we highly recommend to use our Strep-Tactin¢ç purification columns or for high-throughput applications our Strep-Well HT Purification Plates instead of performing batch purification with MagStrep. In General One-STrEP-tag leads to better results in batch purification due to its higher affinity to Strep-Tactin¢ç.

Available Formats:

MagStrep "type2" Beads and the MagStrep "type2HC" Beads with higher Strep-tag¢ç binding
capacity compared to MagStrep "type2".

Specifications of MagStrep Beads

 
MagStrep "type 2"
MagStrep "type 2HC"
Bead size
0.5-1.5 µm
0.5-1.5 µm
Magnetite content
20 - 40 %
20 - 40 %
Binding capacity Strep-tag¢ç II fusion proteins:
0.25 -0.5 nmol/mg beads
0.5 -1.0 nmol/mg beads
Form
50 mg/ml suspension in Dulbecco¢¥s PBS;
0.1 % bovine serum albumin;
0.02 % NaN3 id.
50 mg/ml suspension in Dulbecco¢¥s PBS;
0.1 % bovine serum albumin;
0.02 % NaN3 id.
Stability
12 months after shipment
12 months after shipment
Storage
4 ¡ÆC, DO NOT FREEZE id
4 ¡ÆC, DO NOT FREEZE id
Shipment
RT id.
RT id.

Magnetic Separator
for 24 reaction tubes (12x 1,5 ml and 12x 2 ml)

 
Proteins were purified from crude E. coli extracts following standard protocol
Lane 1 : Marker
Lane 2-4: Strep-tag¢ç II proteins purified with MagStrep "type2"
Lane 5-7: Strep-tag¢ç II proteins purified with MagStrep "type2HC"

Specifications of Mag-Strep Magnetic Beads

Binding capacity
Type 2: 300 pmol/mg
Type 2HC: 0.5-1.0 nmol/mg
Support
/
Bead size
Type 2: 0.5-1.5 µm
Linear flow rate
/
Form
50 mg/ml suspension in Dulbecco's PBS;
0.1% bovine serum albumin;
0.02% NaN3
Stability
n.d.
Storage
4 ¡ÆC, DO NOT FREEZE
Shipment
RT
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