6xHistidine-tag antibody

The following antibodies for 6xHistidine-tag detection are offered:

Description	Anti 6xHistidine-tag monoclonal antibody; purified by affinity chromatography
Subclass	IgG1
Specificity	6xHistidine-tag; the antibody was produced in mouse using 6xHistidine-tag linked to PAX6 transcription factor as immunogen
Detection	N- or C-terminal or internal 6xHistidine-tag
Form	Lyophilized from NH₄HCO₃, contains 10 mg sucrose per mg protein
Reconstitution	Reconstitute in phosphate buffered saline (PBS) at a concentration of 0.2 – 0.1 mg/ml (i. e. dissolve with 0.5 – 1.0 ml PBS, respectively). Aliquot and store at -20 °C.
Dilutions	Recommended dilution for detection of 6xHistidine-tag fusion proteins in Western blots (chromogenic detection): 100-200 ng/ml diluted in PBS containing 0.2 % BSA Secondary antibody: rabbit anti-mouse pAb, HRP conjugated; Cat. No. 2 1591-001
Amount	100 µg; sufficient to develop 50-100 Western Blots using 10 ml incubation volume each
Stability	12 months after shipping
Storage	-20 °C
Shipment	RT

Description	Purified polyclonal rabbit anti mouse immunoglobulin fraction conjugated to horseradish peroxidase (HRP)
Specificity	The antibody used for conjugation has not been absorbed to remove cross-reactions to immunoglobulin from other species. Therefore the conjugate shows reactions with mouse immunoglobulin of all classes
Form	Dissolved in 50 mM Tris/HCl 15 mM NaN₃, pH 7.2
Concentration of conjugate	1.3 mg/ml
Dilutions	Dilute 1:1000 for chromogenic detection of bound mouse monoclonal antibodies in Western Blots Dilute 1:2000 (or titrate up to 1:10,000) for detection of mouse monoclonal antibodies in microplate assays
Amount	0.5 ml
Stability	6 months after shipping
Storage	2 - 8 °C
Shipment	RT