UPGRADE NOW to MyTaq¢â Red Mix

 

Convenient pre-mixed, pre-optimized 2x solutions Reduced risk of contamination Dramatically decreases the time required for reaction set-up Reproducible results Direct gel loading

 

Routine PCR applications Products suitable for TA cloning High throughput

 

BioMix¢â Red is a complete ready-to-use 2x reaction mix containing an ultra-stable Taq DNA polymerase. It contains an additional inert red dye that permits easy visualization and direct loading onto a gel. There is no need to add loading buffer as the mix is of sufficiently high density to sink to the bottom of the gel. The red dye migrates like a 350bp fragment on a 2% agarose TAE gel (or 600bp on a 1% agarose).

BioMix Red has been developed to perform PCR assays of many common genomic and cDNA templates; the user has simply to add water, template and primers. It dramatically reduces the time required to set up reactions, thereby minimizing the risk of contamination. Greater reproducibility is ensured by reducing the number of pipetting steps that can lead to errors.

BioMix Red has been optimized for a wide variety of templates, however an additional 50mM of MgCl₂ solution is included should any fine adjustments be required.

 

 
BioMix Red 50mM MgCl₂ Solution

 

BIO-25005: 100 x 50¥ìl Reactions: 2 x 1.25ml
BIO-25006: 500 x 50¥ìl Reactions: 10 x 1.25ml

 

2x

 

BioMix Red can be stored for up to 12 months at -20¡ÆC, or up to 2 weeks at +4¡ÆC. Repeated freeze/thaw cycles should be avoided.

 

Coy, S., et al. Mol. Cell. Biol. 31, 1432-1443 (2011). O'Hara, L., et al. Endo. 152, 718-729 (2011). Kapralov, M.V. et al. Mol. Biol. Evol. 28, 1491-1503 (2011). Wang, X. et al. J. Exp. Bot. 62, 2973-2987 (2011). Perez-Brocal, V. et al. Genome Biol. Evol. 2, 257-266 (2010). Kayani, A.C., et al. Am. J. Physiol. 299, R268-R276 (2010). Stephane, B., et al. Plant Physiol. 154, 1966-1982 (2010). Robbins, J.C. et al. RNA 15, 1142-1153 (2009). Schultz, J.K. et al. J. Hered. 100, 25-33 (2009). Welsh, M., et al. FASEB J. 23, 4218-4230 (2009). Rayner, B.S., et al. J. Neurochem. 97(1), 211 (2006).

 

BioMix is a trademark of Bioline.