SensiMix SYBR¢ç Low-ROX Kit is designed for superior sensitivity and specificity on various real-time instruments in which a ROX signal is required. The mix has been developed to provide the high sensitivity required for demanding assays or for those assays where template material is limited.
Features & BenefitsSensitivity - allows reproducible detection of low-copy number templates
Reproducibility - generates real-time data reliably and reproducibly
Specific - proprietary hot-start chemistry minimises non-specific amplification
Broad dynamic range - a dynamic range of up to eight orders of magnitude
Instrument CompatibilityABI 7500, Stratagene Mx4000¢ç, Mx3000P¢ç and Mx3005P¢ç. (See product selection table). However, the kit is also compatible with several instruments that do not require the use of ROX¢â, such as the Qiagen Rotor-Gene¢â Q, the Bio-Rad CFX96 or the Roche LightCycler¢ç 480.
DescriptionSensiMix SYBR Low-ROX kit is a pre-mixed, high-performance product designed for superior sensitivity and specificity (fig. 1) on various real-time instruments in which a ROX signal is required, particularly for those block-based platforms which are more sensitive to the dye levels.
Powered by a proprietary highly optimized, chemical hot-start PCR enzyme, the mix has been developed to provide the high sensitivity and specificity required for demanding assays or for those assays where template material is limited.
SensiMix SYBR Low-ROX is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR¢ç Green I dye, dNTPs, stabilisers and enhancers. NotesSensiMix is a trademark of Bioline Reagents Ltd. protocol [pi_50232_sensimix_sybr_low_rox_kit_v1.pdf] (435,286kb) |
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