*2019³â ÀÌÈÄ Ãë±Þ ÇÏÁö ¾ÊÀ½CopyRight¢ç v2.0 Fosmid Cloning Kits (pSMART¢ç FOS)
Improved Fosmid CloningLucigen¡¯s CopyRight¢ç v2.0 Fosmid Cloning Kit with the pSMART¢ç FOS vector and Replicator¢â FOS strain contains the most advanced fosmid cloning developed. The CopyRight v2.0 Fosmid Kit yields many more recombinants from limited starting DNA (Figure 1). The vector incorporates CopyRight transcription terminators to stabilize inserts, which results in complete fosmid libraries without missing clones. False-negatives and false-positives are dramatically reduced, and empty-vector background is essentially eliminated (Figure 2). Controllable genetic elements in the pSMART FOS vector (Figure 3) and Replicator FOS cells allow amplification of vector copy number for high plasmid yields (Figure 4).
Convenient SuccessEfficiently obtain your clone or create your library—the CopyRight v2.0 Cloning Kits eliminate tedious vector and competent cell preparation, as well as time-consuming QC testing experiments. Kits include optimized reagents, ligation-ready vector, optimized cells, detailed instructions, and trouble-shooting guide.
Replicator FOS genotype: F¯ mcrA ¥Ä(mrr-hsdRMS-mcrBC) endA1 recA1 ¥Õ80dlacZ¥ÄM15 ¥ÄlacX74 ara¥Ä139 ¥Äara,leu)7697 galU galK rpsL nupG (attL araC-PBAD-trfA250 bla attR) ¥ë-.
MA027-CopyRight2-pSMART-FOS.pdf (537,648kb) MSDS.pdf (118,131kb) eLucidations_v7.pdf (1,915,087kb) Newsletter_vol3.pdf (1,299,166kb) |
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