Label IT¢ç miRNA Labeling Kits
CAT# PRODUCT SIZE STORAGE ¼ÒºñÀÚ°¡ ÁÖ¹®
MIR 9410Label IT¢ç miRNA Labeling Kit, Biotin10 reactions-20¡ÆC-·Î±×ÀÎ
MIR 9450Label IT¢ç miRNA Labeling Kit, Biotin50 reactions-20¡ÆC-·Î±×ÀÎ
MIR 9305Label IT¢ç miRNA Labeling Kit, Cy¢ç3/Cy¢ç52 x 5 reactions-20¡ÆC-·Î±×ÀÎ
MIR 9325Label IT¢ç miRNA Labeling Kit, Cy¢ç3/Cy¢ç52 x 25 reactions-20¡ÆC-·Î±×ÀÎ
MIR 9510Label IT¢ç miRNA Labeling Kit, Cy¢ç310 reactions-20¡ÆC-·Î±×ÀÎ
MIR 9550Label IT¢ç miRNA Labeling Kit, Cy¢ç350 reactions-20¡ÆC-·Î±×ÀÎ
MIR 9610Label IT¢ç miRNA Labeling Kit, Cy¢ç510 reactions-20¡ÆC-·Î±×ÀÎ
MIR 9650Label IT¢ç miRNA Labeling Kit, Cy¢ç510 reactions-20¡ÆC-·Î±×ÀÎ
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Label IT¢ç miRNA Labeling Kits

Rapid and sensitive non-enzymatic labeling of microRNA (miRNA) in total or enriched RNA samples for microarray analysis

* Sensitive - Detects subattomolar amounts of miRNA species.

* Accurate - Labels all miRNAs present in the sample.

* Universal - Labels total or enriched RNA from fresh, frozen or FFPE tissues including plants.

* Sequence Independent Labeling - Labels all nucleotides with equal efficiency.

* Save Time - Simple, one hour protocol.


Kit Components:
Each kit includes the Label IT¢ç miRNA Labeling Reagents, Reconstitution Solution, 10X Labeling Buffer M, 10X Stop Solution, Purification Columns (with Collection Tubes), Column Binding Buffer, Column Wash Buffer, Column Elution Solution, and Elution Tubes. This kit also contains a 2X Hybridization Solution.



Figure 1. The Label IT¢ç miRNA Labeling Kits. The Label IT¢ç Cy¢ç3, Cy¢ç5, and biotin reagents are composed of three regions: the Cy¢ç3, Cy¢ç5 or biotin label (green), the linker (yellow) which facilitates electrostatic interactions with nucleic acids and the reactive alkylating group (blue) that covalently attaches the Label IT¢ç reagents to any reactive heteroatom within miRNAs. The covalent attachment of the Label IT¢ç reagents to miRNA does not alter the structure or downstream hybridization performance of the miRNA, and thus, once labeled, the miRNA samples can be hybridized to miRNA-specific microarrays and analyzed to determine the miRNA expression profile.



Figure 2. The Label IT¢ç miRNA Labeling Kit is More Sensitive Than NCode¢â. A pool of 699 synthetic miRNAs was labeled and hybridized to the NCode¢â Multi-Species miRNA Microarray V2 at varying input amounts. Features with signal greater than 3X background were considered detected.



Figure 3. Accurately Detect All miRNAs in Your Sample. Representative array images of specific miRNAs missed by enzymatic labeling. Yellow tests were performed using synthetic pools of miRNAs (2.5 fmol of each miRNA) labeled with either chemical (Label IT¢ç) or enzymatic (NCode¢â Rapid) labeling, and hybridized to the NCode¢â Multi-Species miRNA Microarray.



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