Label IT® Tracker™ Intracellular Nucleic Acid Localization Kits

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Description

CAT#	PRODUCT	SIZE	STORAGE	소비자가	주문
MIR 7024	Label IT® Tracker™ Intracellular Nucleic Acid Localization Kit, Biotin	labels 50-200 µg plasmid DNA	-20°C	-	로그인
MIR 7022	Label IT® Tracker™ Intracellular Nucleic Acid Localization Kit, CX-Rhodamine	labels 50-200 µg plasmid DNA	-20°C	-	로그인
MIR 7020	Label IT® Tracker™ Intracellular Nucleic Acid Localization Kit, Cy®3	labels 50-200 µg plasmid DNA	-20°C	-	로그인
MIR 7021	Label IT® Tracker™ Intracellular Nucleic Acid Localization Kit, Cy®5	labels 50-200 µg plasmid DNA	-20°C	-	로그인
MIR 7023	Label IT® Tracker™ Intracellular Nucleic Acid Localization Kit, TM-Rhodamine	labels 50-200 µg plasmid DNA	-20°C	-	로그인
MIR 7025	Label IT® Tracker™ Intracellular Nucleic Acid Localization Kit, Fluorescein	labels 50-200 µg plasmid DNA	-20°C	-	로그인
				-	로그인

Label IT® Tracker™ Intracellular Nucleic Acid Localization Kits

Efficient, direct labeling of plasmid DNA for in vitro and in vivo tracking experiments

Technical Reports

Intracellular Localization and Expression of Labeled Plasmid DNA Using Label IT® Tracker™ Nucleic Acid Labeling Kits (pdf)
Nucleic Acid Labeling and Delivery Tools for Neurobiology (pdf)

Versatile Labeling - Efficiently label and visualize the DNA of your choice.
One-step Chemical Method - Easily and consistently control the labeling reactions.
High Efficiency Labeling - Optimal visualization of DNA in cells.
Superior Tracking - Monitor both subcellular localization and reporter transgene expression following transfection of labeled (plasmid) DNA.

Kit Components:
Each Kit contains Label IT® Tracker™ Reagent, Label IT® Reconstitution Solution, and 10X Labeling Buffer.

Figure 1. The Label IT® Tracker™ Kits. The Label IT® Tracker™ labeling reagents are composed of three regions: the label (a fluorophore or biotin) (green), the linker (yellow) which facilitates electrostatic interactions with plasmid DNA and the reactive alkylating group (blue) that covalently attaches the Label IT® reagents to any reactive heteroatom within the plasmid. Attachment of the Label IT® Reagent to plasmids does not alter their structure or affect downstream gene expression, and as such, the same labeled plasmid can be both visualized after transfection by fluorescence microscopy and employed to express the gene of interest on the plasmid.

Figure 2. Tracking of Plasmid Localization and Expression. COS-7 cells transfected with Label IT® Tracker™ Cy®5 (blue) labeled pEYFP-nuc and TransIT®-LT1 Reagent in serum containing media. Images were acquired at 3, 8, and 20 hours post-transfection. The blue signal indicates the cellular localization of the labeled plasmid, the green signal indicates cellular autofluorescence, and the yellow signal is the expression of the nuclear yellow fluorescent protein (YFP) reporter.

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