Probe-Based qPCR Master Mix
CAT# PRODUCT SIZE STORAGE Һڰ ֹ
10557701 mL PrimeTime Gene Expression Master Mix1 x 1 mL-20C-α
10557725 mL PrimeTime Gene Expression Master Mix1 x 5 mL-20C-α
105577125 mL PrimeTime Gene Expression Master Mix5 x 5 mL-20C-α
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Probe-Based qPCR Master Mix

PrimeTime Gene Expression Master Mix probe-based qPCR assays ȭ Ǿ ְ, two-step RT-qPCR >90% ȿ մϴ.


 Benefits


1. Two-step RT-qPCR master mix
2. Fast standard cycling conditions
3. Multiplex 迡 ΰ sensitivity
4. temperature stability : 4~8ð 55C ص efficiency
5. , probe ȣȯ
6.  master mix ȿ
7. Batch-to-Batch consistence :  24ð variation ȿ ״


Consist of :
2X master mix solution  : (antibody-mediated, hot-start DNA polymerase; dNTPs; MgCl2; enhancers; and stabilizers)
separate reference dye stock solution ( Thermo science, Agilent, AB )

As good or better than other commercial master mixes


PrimeTime Gene Expression Master Mix delivers consistent results that are as good or better than the other commercially available master mixes tested. Cq values using the IDT master mix were approximately 2–4 Cqs earlier in this representative experiment.


IDT qPCR master mix is as good or better than others

Figure 1. PrimeTime Gene Expression Master Mix delivers consistent results that are as good or better than 7 other commercially available master mixes tested. PCRs contained PrimeTime HPRT qPCR Assays, PrimeTime Gene Expression Master Mix with reference dye, and either cDNA or gBlocks Gene Fragments as template. PCRs were run in triplicate on a 7900HT Real-Time PCR System (Thermo Fisher Scientific).



Standard or fast cycling conditions

PrimeTime Gene Expression Master Mix performs well under standard or fast cycling conditions (Figures 1–2) on a variety of real-time qPCR instruments, including the 7900HT Real-Time (Thermo Fisher Scientific), QuantStudio 7 Flex (Thermo Fisher Scientific), CFX384 (BioRad), and LightCycler 480 (Roche) qPCR systems.

PCR efficiency distribution (standard and fast cycling)

Figure 4. High PCR efficiency under standard or fast cycling conditions. PCRs consisting of PrimeTime qPCR Assays, PrimeTime Gene Expression Master Mix, reference dye, and template were run on a 7900HT Real-Time PCR System (Thermo Fisher Scientific) using standard or fast cycling conditions. Standard cycling conditions: 3 min. 95C; 49 x (15 sec. 95C; 1 min. 60). Fast cycling conditions: 3 min. 95C; 49 x (5 sec. 95C; 30 sec. 60). This histogram shows the calculated PCR efficiency of 13 assays run under standard (orange) or fast (blue) cycling conditions using either diluted cDNA (0.016–50 ng) or gBlocks Gene Fragments (101–107 copies) as template. All assays exhibited 90–110% PCR efficiency with R2 >0.99.


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