Probe-Based qPCR Master Mix
CAT# PRODUCT SIZE STORAGE 소비자가 주문
10557701 mL PrimeTime® Gene Expression Master Mix1 x 1 mL-20°C-로그인
10557725 mL PrimeTime® Gene Expression Master Mix1 x 5 mL-20°C-로그인
105577125 mL PrimeTime® Gene Expression Master Mix5 x 5 mL-20°C-로그인
-로그인

Probe-Based qPCR Master Mix 출시

PrimeTime® Gene Expression Master Mix 는 probe-based qPCR assays에 최적화 되어 있고, two-step RT-qPCR 에서 >90% 의 효율을 보장 합니다.


 Benefits


1. Two-step RT-qPCR master mix
2. Fast 와 standard cycling conditions 에 모두 사용 가능
3. Multiplex 실험에서도 민감한 sensitivity
4. temperature stability : 4~8시간 55°C에 보관해도 efficiency 유지
5. 모든 장비, probe에 호환 가능
6. 기존의 어떤 master mix 보다 좋은 효율
7. Batch-to-Batch consistence :  24시간 상온 보관에도 variation이 없이 효율이 그대로 유지


Consist of :
2X master mix solution  : (antibody-mediated, hot-start DNA polymerase; dNTPs; MgCl2; enhancers; and stabilizers)
separate reference dye stock solution ( Thermo science, Agilent, AB 장비용)

As good or better than other commercial master mixes


PrimeTime® Gene Expression Master Mix delivers consistent results that are as good or better than the other commercially available master mixes tested. Cq values using the IDT master mix were approximately 2–4 Cqs earlier in this representative experiment.


IDT qPCR master mix is as good or better than others

Figure 1. PrimeTime® Gene Expression Master Mix delivers consistent results that are as good or better than 7 other commercially available master mixes tested. PCRs contained PrimeTime HPRT qPCR Assays, PrimeTime Gene Expression Master Mix with reference dye, and either cDNA or gBlocks® Gene Fragments as template. PCRs were run in triplicate on a 7900HT Real-Time PCR System (Thermo Fisher Scientific).



Standard or fast cycling conditions

PrimeTime® Gene Expression Master Mix performs well under standard or fast cycling conditions (Figures 1–2) on a variety of real-time qPCR instruments, including the 7900HT Real-Time (Thermo Fisher Scientific), QuantStudio™ 7 Flex (Thermo Fisher Scientific), CFX384 (BioRad), and LightCycler® 480 (Roche) qPCR systems.

PCR efficiency distribution (standard and fast cycling)

Figure 4. High PCR efficiency under standard or fast cycling conditions. PCRs consisting of PrimeTime® qPCR Assays, PrimeTime Gene Expression Master Mix, reference dye, and template were run on a 7900HT Real-Time PCR System (Thermo Fisher Scientific) using standard or fast cycling conditions. Standard cycling conditions: 3 min. 95°C; 49 x (15 sec. 95°C; 1 min. 60°). Fast cycling conditions: 3 min. 95°C; 49 x (5 sec. 95°C; 30 sec. 60°). This histogram shows the calculated PCR efficiency of 13 assays run under standard (orange) or fast (blue) cycling conditions using either diluted cDNA (0.016–50 ng) or gBlocks® Gene Fragments (101–107 copies) as template. All assays exhibited 90–110% PCR efficiency with R2 >0.99.


사용 후기
사용후기가 없습니다.
사용후기 작성
관련제품
PrimeTime® qPCR 5' Nuclease Assays (Probe)
PrimeTime® qPCR Probes and Quenchers
gototop