Ultramer RNA Oligos (up to 120 bases)

Ultramer¢ç RNA Oligo ¿Ã¸®°í ÇÕ¼º ¼­ºñ½º

IDT´Â ³ôÀº Ç°ÁúÀÇ ±ä RNA oligo(~ÃÖ´ë 120mer)¸¦ ÇÕ¼ºÇϱâ À§ÇØ ÃÖÀûÈ­µÈ ±â¼ú·ÂÀ¸·Î Ultramers¢ç RNA oligonucleotide¸¦ Á¦°øÇÕ´Ï´Ù. ÀÌ single stranded custom RNA´Â ´ÙÀ½ÀÇ RNA°ü·Ã ´Ù¾çÇÑ applications¿¡  Çâ»óµÈ specificity¿Í  performance¸¦ Á¦°øÇÒ ¼ö ÀÖ½À´Ï´Ù.

  • Unmodified single-guide RNAs(sgRNAs) for CRISPR

  • Long RNAs for RNA therapeutics development

  • RNA controls for PCR and qPCR

Ultramers¢ç RNA´Â ÁÖ¹® ÈÄ ¾à 7-10ÀÏ ÈÄ¿¡ desalting °úÁ¤À¸·Î ºÒ¼ø¹°À» Á¦°ÅÇÑ µÚ¿¡ 4 nmol, 20 nmol ¶Ç´Â 80 nmol·Î Á¤·®µÈ ´ÙÀ½ °ÇÁ¶µÈ »óÅ·Πtube¿¡ ³Ö¾îÁ® ¹è¼ÛµË´Ï´Ù. Á¦Ç°°ú ÇÔ²² ESI mass spectrometry QC ÀڷḦ ÇÔ²² ¹ÞÀ» ¼ö ÀÖ½À´Ï´Ù.

 

IDT´Â RNA ÇÕ¼ºÀÇ ºü¸£°í ³ôÀº fidelity Çâ»óÀ» À§ÇØ Á¦Á¶ °úÁ¤À» Çõ½ÅÇÏ¿© IDT ÀÚüÀÇ solid supportÀÇ pairingÀ¸·Î ÃÖÀûÈ­µÈ RNA ÇÕ¼º ±â¼úÀ» º¸À¯ÇÏ°í ÀÖ½À´Ï´Ù. IDTÀÇ R&D ÆÀÀº ¼º°øÀûÀ¸·Î ±ä RNAÀÇ ÇÕ¼ºÀ» À§ÇØ RNA oligoÀÇ coupling efficiency¸¦ 99.0-99.3%±îÁö Çâ»óÇÏ¿© Ultramers¢ç RNA·Î Á¦°øÇÕ´Ï´Ù.

Product Specifications:  

  • 60 ¿¡¼­120 bases ±îÁö ÇÕ¼º
  • 4 nmole, 20 nmole, 80 nmole ·Î Á¤·®µÇ¼­ dry »óÅ·Π¹è¼Û
  • CRISPR sgRNA, PCR ¶Ç´Â qPCRÀÇ control, RNA Ä¡·áÁ¦ °³¹ßÀ» À§ÇÑ Long RNA·Î ÀûÇÕ 
  • Standard desalted Ultramers¢ç ÀÇ ¹è¼ÛÀº ¾à 7-8ÀÏ Á¤µµ ¼Ò¿ä  


Product


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4 nmole Ultramer¢ç RNA Oligo


Standard Desalt

4 nmole

60 - 120 Bases

20 nmole Ultramer¢ç RNA Oligo

Standard Desalt

20 nmole

60 - 120 Bases

80 nmole Ultramer¢ç RNA Oligo


Standard Desalt

80 nmole

60 - 120 Bases


Ultramer¢ç RNA ¼­ºñ½º¿¡ °¡´ÉÇÑ Modificaions.


Product

4 nmole Ultramer¢ç RNA Oligo

20 nmole Ultramer¢ç RNA Oligo

80 nmole Ultramer¢ç RNA Oligo

5' 6-FAM


5' Amino Modifier C12

5' Amino Modifier C6


5' Biotin


5' deoxyInosine


5' deoxyUridine



coupling efficiency ranges - Ultramer RNA oligonucleotides

 
Figure 1. The general relationship between percentage of full-length product, coupling efficiency, and oligo length. Coupling efficiency becomes increasingly important with increased oligo length. In long oligos, small changes in coupling efficiency have dramatic effects on full-length yield. The graphs depicts the difference in full-length product for coupling efficiencies of 99.0–99.3% (dark blue) and 98.0–98.5% (light blue)
 RNA Ultramer fig. 2B

Figure 2B. QC data demonstrates high purity of a 120mer Ultramer¢ç RNA Oligo. The measured molecular weight of the oligo is within 0.01% of its expected molecular weight, which is determined by the specific oligo sequence. Data generated from ESI-MS instruments at IDT.

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