Ultramer RNA Oligos (up to 120 bases)

Ultramer RNA Oligo ø ռ

IDT ǰ RNA oligo(~ִ 120mer) ռϱ ȭ Ultramers RNA oligonucleotide մϴ. single stranded custom RNA RNA پ applications   specificity  performance ֽϴ.

  • Unmodified single-guide RNAs(sgRNAs) for CRISPR

  • Long RNAs for RNA therapeutics development

  • RNA controls for PCR and qPCR

Ultramers RNA ֹ 7-10 Ŀ desalting Ҽ ڿ 4 nmol, 20 nmol Ǵ 80 nmol · tube ־ ۵˴ϴ. ǰ Բ ESI mass spectrometry QC ڷḦ Բ ֽϴ.

 

IDT RNA ռ fidelity Ͽ IDT ü solid support pairing ȭ RNA ռ ϰ ֽϴ. IDT R&D RNA ռ RNA oligo coupling efficiency 99.0-99.3% Ͽ Ultramers RNA մϴ.

Product Specifications:  

  • 60 120 bases ռ
  • 4 nmole, 20 nmole, 80 nmole  Ǽ dry ·
  • CRISPR sgRNA, PCR Ǵ qPCR control, RNA ġ  Long RNA  
  • Standard desalted Ultramers 7-8 ҿ  


Product

( base )

4 nmole Ultramer RNA Oligo

8,400

Standard Desalt

4 nmole

60 - 120 Bases

20 nmole Ultramer RNA Oligo

12,600

Standard Desalt

20 nmole

60 - 120 Bases

80 nmole Ultramer RNA Oligo

22,400

Standard Desalt

80 nmole

60 - 120 Bases


Ultramer RNA 񽺿 Modificaions.


Product

4 nmole Ultramer RNA Oligo

20 nmole Ultramer RNA Oligo

80 nmole Ultramer RNA Oligo

5' 6-FAM

91,000

91,000

113,400

5' Amino Modifier C12

77,000

77,000

98,000

5' Amino Modifier C6

36,400

36,400

44,800

5' Biotin

63,000

63,000

77,000

5' deoxyInosine

14,000

14,000

16,800

5' deoxyUridine

14,000

14,000

16,800

5' Phosphorylation

28,000

28,000

35,000


coupling efficiency ranges - Ultramer RNA oligonucleotides

 
Figure 1. The general relationship between percentage of full-length product, coupling efficiency, and oligo length. Coupling efficiency becomes increasingly important with increased oligo length. In long oligos, small changes in coupling efficiency have dramatic effects on full-length yield. The graphs depicts the difference in full-length product for coupling efficiencies of 99.0–99.3% (dark blue) and 98.0–98.5% (light blue)
 RNA Ultramer fig. 2B

Figure 2B. QC data demonstrates high purity of a 120mer Ultramer RNA Oligo. The measured molecular weight of the oligo is within 0.01% of its expected molecular weight, which is determined by the specific oligo sequence. Data generated from ESI-MS instruments at IDT.

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