The success of next-generation sequencing is dependent upon the precise and accurate processing of the input DNA. This requires high-quality library preparation using a coordinated series of standard molecular biology reactions whilst maintaining high yields during the intermediate purification steps.

The JetSeq¢â ER and Ligation Kit is designed to generate high-quality PCR-free, next generation sequencing (NGS) libraries making it ideal for whole-genome sequencing applications on Illumina^¢ç MiniSeq¢â, MiSeq¢â, NextSeq¢â or HiSeq¢â instruments. The kit contains all of the enzymes and buffers necessary for end-repair, A-tailing and ligation in a convenient master mix formulation. It offers a fast, streamlined workflow and flexibility where users are free to adapters of their choice.

By combining end-repair and A-tailing in one unique step, the JetSeq ER and Ligation Kit is able to reduce total NGS library preparation time and minimize the variability caused by additional handling, as well as the risk of contamination and material lose.