The success of next-generation sequencing is dependent upon the precise and accurate processing of the input DNA. This requires high-quality library preparation using a coordinated series of standard molecular biology reactions whilst maintaining high yields during the intermediate purification steps.

The JetSeq¢â Flex DNA Library Preparation Kit is designed to generate high-quality next generation sequencing (NGS) libraries, even with more limited amounts of starting material and is suitable for sequencing on Illumina^¢ç MiniSeq¢â, MiSeq¢â, NextSeq¢â or HiSeq¢â instruments. The kit contains all of the enzymes and buffers necessary for end-repair, A-tailing, ligation and PCR amplification in convenient master mix formulations. It offers a fast, streamlined workflow and flexibility where users are free to adapters of their choice.

By combining end-repair and A-tailing in one unique step, the JetSeq Flex DNA Library Preparation Kit is able to reduce total NGS library preparation time and minimize the variability caused by additional handling, as well as the risk of contamination and material lose. A high quality DNA polymerase is also supplied to selectively enrich library fragments carrying the adapter sequences and to amplify the amount of DNA prior to sequencing.