PrimeTime¢ç qPCR Probes and Quenchers

PrimeTime¢ç qPCR Probes

ÀÏ¹Ý assay¿¡ »ç¿ëµÇ´Â dual labeled probes´Â target sequence¿¡ hybridizationµÈ ÈÄ nuclease degradation¿¡ ÀÇÇØ reporter Çü±¤ÀÌ quencher·ÎºÎÅÍ ºÐ¸®µÇ¾î ±× ºûÀ» ³»µµ·Ï °í¾ÈµÈ probeÀÔ´Ï´Ù. ´ëºÎºÐÀÇ dual-labeled probe´Â real-time PCR assay, end-point hybridization assay,¶Ç´Â DNase¿Í RNaseÀÇ detectionÀ» À§ÇÑ substrate·Î½á ȤÀº DNA ±¸Á¶¿¡¼­ protein-induced change ÃøÁ¤ assay¿¡¼­ »ç¿ëµË´Ï´Ù. ÀüÇüÀûÀÎ reporter¿Í quencherÀÇ °Å¸®´Â ¾à 15-35 bases°¡ ÀûÀýÇÕ´Ï´Ù.

PrimeTime¢ç Mini qPCR Probes

PrimeTime Mini probes´Â ÀÛÀº ½ºÄÉÀÏÀÇ dual-labeled probes ÀÔ´Ï´Ù. º¸´Ù ÀÛÀº ½ºÄÉÀÏ°ú Àú·ÅÇÑ °¡°ÝÀ¸·Î ¿©·¯ À¯ÀüÀÚ¿¡ ´ëÇؼ­ »õ·Î¿î probe¸¦ Å×½ºÆ®Çϴµ¥ ÀÌ»óÀûÀÔ´Ï´Ù.
¸ðµç Mini probes´Â 5' FAM °ú 3' IBFQ ¿Í internal ZEN¢â quencher Á¶ÇÕÀ¸·Î µÇ¾î ÀÖÀ¸¸ç ¾à 1ÁÖÀÏ Á¤µµ ¼Ò¿äµË´Ï´Ù.

PrimeTime¢ç Eco qPCR Probes

The PrimeTime¢ç Eco qPCR Probe´Â 500 reactionÀÌÇÏÀÇ À¯ÀüÀÚ ¹ßÇö ºÐ¼®À» ÇÏ´Â ¿¬±¸Àڵ鿡°Ô ¸Å¿ì ÀÌ»óÀûÀÎ scaleÀÔ´Ï´Ù. Áß°£ scale°ú ³·Àº °¡°ÝÀº ¸¹Àº »ùÇà ¼ÂÆ®¸¦ Ãʱâ screening Çϴµ¥ ¸Å¿ì ÀûÀýÇÕ´Ï´Ù. Eco qPCR Probes´Â 5' FAM °ú 3' IBFQ ¿Í internal ZEN¢â quencher Á¶ÇÕÀ¸·Î µÇ¾î ÀÖÀ¸¸ç ¾à 1ÁÖÀÏ Á¤µµ ¼Ò¿äµË´Ï´Ù.

PrimeTime¢ç Mini qPCR Probes º¸Àå·®

 
5' Reporter Dye(s)
Quencher(s)
Delivery Amount

FAM
ZEN / Iowa Black FQ *
0.5 nmoles

 

PrimeTime¢ç Eco qPCR Probes º¸Àå·®

Reporter Dye(s)
Quencher(s)
Delivery Amount

FAM
ZEN / Iowa Black FQ *
2.5 nmoles

 
 

PrimeTime¢ç qPCR Probes Standard level º¸Àå·®

5' Reporter Dye(s)
Quencher
100 nmole / 10 nmoles
250 nmole / 25 nmoles
1 umole / 50 nmoles

FAM
 
ZEN / Iowa Black FQ *


 TAMRA
 
 Black Hole Quencher 1

HEX
ZEN / Iowa Black FQ *

YAK

ZEN / Iowa Black FQ *

N/A


TET
Black Hole Quencher 1

 
 

PrimeTime¢ç qPCR Probes Complex level º¸Àå·®

 
5' Reporter Dye(s)
Quencher
100 nmole / 2 nmoles
250 nmole / 8 nmoles
1 umole / 20 nmoles
FAM
TAMRA NHS Ester
N/A

MAX NHS¡¡
ZEN / Iowa Black FQ *

Black Hole Quencher 1

Cy3
Iowa Black RQ¡¡¡¡¡¡
            
¡¡¡¡
Cy5

 Cy5

 TAO / Iowa Black RQ

TEX 615

 Iowa Black RQ¡¡

 

¡¡¡¡¡¡

TYE 563
TYE 665
Black Hole Quencher 2

JOE NHS Ester¡¡
ZEN / Iowa Black FQ *
N/A

Black Hole Quencher 1
N/A

TAMRA NHS Ester
Iowa Black RQ¡¡
N/A¡¡

ROX NHS Ester
Texas Red-X NHS Ester
Black Hole Quencher 2
N/A

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