Affinity Plus qPCR Probes
Increase SNP target specificity and use shorter sequences with these high Tm probes
Use Affinity Plus qPCR Probes for SNP genotyping, transcript variant identification, and more sensitive target detection in challenging samples (FFPE tissue, biofluids). The Affinity Plus bases used in these qPCR probes include up to 6 locked nucleic acid monomers (see the technology page, Locked nucleic acids, for more information). When incorporated into a probe, they impart heightened structural stability, leading to increased hybridization melt temperature (Tm).
- Improve mismatch discrimination compared to traditional qPCR probes
- Benefit from this affordable approach to increasing assay hybridization specificity
- Make flexible Tm adjustments more easily than possible with MGB probes
Ordering
Mini Affinity Plus qPCR Probes
Mini Affinity Plus qPCR Probes are ideal for screening small sample sets or performing just a few reactions when optimizing probe designs.
5' Reporter Dye(s) |
Quencher(s) |
Delivery Amount |
0.5 nmol |
FAM |
Iowa Black FQ * |
|
HEX |
Iowa Black FQ * |
|
YAK |
Iowa Black FQ * |
| | | |
Affinity Plus qPCR Probes
Affinity Plus qPCR Probes are offered with a wider selection of dyes and quenchers than Affinity Plus Mini qPCR Probes, and are best-suited for large-scale or high-throughput applications.
5' Reporter Dye |
Quencher |
Synthesis Scale |
Min Guarantee |
|
5' 6-FAM¢â |
3' Black Hole Quencher¢ç 1 |
1 µmole |
20 nmoles |
|
250 nmole |
8 nmoles |
|
3' Iowa Black¢ç FQ |
1 µmole |
20 nmoles |
|
250 nmole |
8 nmoles |
|
5' Reporter Dye |
Quencher |
Synthesis Scale |
Min Guarantee |
|
5' Cy3¢â |
3' Black Hole Quencher¢ç 2 |
1 µmole |
20 nmoles |
|
250 nmole |
8 nmoles |
|
3' Iowa Black¢ç RQ-Sp |
1 µmole |
20 nmoles |
|
250 nmole |
8 nmoles |
|
5' Reporter Dye |
Quencher |
Synthesis Scale |
Min Guarantee |
|
5' Cy5¢â |
3' Black Hole Quencher¢ç 2 |
1 µmole |
20 nmoles |
|
250 nmole |
8 nmoles |
|
3' Iowa Black¢ç RQ-Sp |
1 µmole |
20 nmoles |
|
250 nmole |
8 nmoles |
|
5' Reporter Dye |
Quencher |
Synthesis Scale |
Min Guarantee |
|
5' TEX 615 |
3' Black Hole Quencher¢ç 2 |
1 µmole |
20 nmoles |
|
250 nmole |
8 nmoles |
|
3' Iowa Black¢ç RQ-Sp |
1 µmole |
20 nmoles |
|
250 nmole |
8 nmoles |
|
5' Reporter Dye |
Quencher |
Synthesis Scale |
Min Guarantee |
|
5' TYE¢â 563 |
3' Black Hole Quencher¢ç 2 |
1 µmole |
20 nmoles |
|
250 nmole |
8 nmoles |
|
3' Iowa Black¢ç RQ-Sp |
1 µmole |
20 nmoles |
|
250 nmole |
8 nmoles |
|
5' Reporter Dye |
Quencher |
Synthesis Scale |
Min Guarantee |
|
5' TYE¢â 665 |
3' Black Hole Quencher¢ç 2 |
1 µmole |
20 nmoles |
|
250 nmole |
8 nmoles |
|
3' Iowa Black¢ç RQ-Sp |
1 µmole |
20 nmoles |
|
250 nmole |
8 nmoles
|
|
5' Reporter Dye |
Quencher |
Synthesis Scale |
Min Guarantee |
|
5' HEX¢â |
3' Black Hole Quencher¢ç 1 |
1 µmole |
20 nmoles |
|
250 nmole |
8 nmoles |
|
3' Iowa Black¢ç FQ |
1 µmole |
20 nmoles |
|
250 nmole |
8 nmoles |
|
5' Reporter Dye |
Quencher |
Synthesis Scale |
Min Guarantee |
|
5' Yakima Yellow¢ç |
3' Iowa Black¢ç FQ |
1 µmole |
20 nmoles |
|
250 nmole |
8 nmoles |
|