Affinity Plus¢ç qPCR Probes
CAT# PRODUCT SIZE STORAGE ¼ÒºñÀÚ°¡ ÁÖ¹®
512591 µm Affinity Plus¢ç 5' 6-FAM¢â / 3' BHQ¢ç-11um-·Î±×ÀÎ
512601 µm Affinity Plus¢ç 5' 6-FAM¢â / 3' IB¢çFQ1um-·Î±×ÀÎ
512611 µm Affinity Plus¢ç 5' Cy3¢â / 3' BHQ¢ç-21um-·Î±×ÀÎ
512621 µm Affinity Plus¢ç 5' Cy3¢â / 3' IB¢çRQ1um-·Î±×ÀÎ
512631 µm Affinity Plus¢ç 5' Cy5¢â / 3' BHQ¢ç-21um-·Î±×ÀÎ
512641 µm Affinity Plus¢ç 5' Cy5¢â / 3' IB¢çRQ1um-·Î±×ÀÎ
512651 µm Affinity Plus¢ç 5' HEX¢â / 3' BHQ¢ç-11um-·Î±×ÀÎ
512661 µm Affinity Plus¢ç 5' HEX¢â / 3' IB¢çFQ1um-·Î±×ÀÎ
512671 µm Affinity Plus¢ç 5' TEX¢â 615 / 3' BHQ¢ç-21um-·Î±×ÀÎ
512681 µm Affinity Plus¢ç 5' TEX¢â 615 / 3' IB¢çRQ1um-·Î±×ÀÎ
512691 µm Affinity Plus¢ç 5' TYE¢â 563 / 3' BHQ¢ç-21um-·Î±×ÀÎ
512701 µm Affinity Plus¢ç 5' TYE¢â 563 / 3' IB¢çRQ1um-·Î±×ÀÎ
512711 µm Affinity Plus¢ç 5' TYE¢â 665 / 3' BHQ¢ç-21um-·Î±×ÀÎ
512721 µm Affinity Plus¢ç 5' TYE¢â 665 / 3' IB¢çRQ1um-·Î±×ÀÎ
512731 µm Affinity Plus¢ç 5' YAK¢ç/ 3' IB¢çFQ1um-·Î±×ÀÎ
51274250 nm Affinity Plus¢ç 5' 6-FAM¢â / 3' BHQ¢ç-1250nm-·Î±×ÀÎ
51275250 nm Affinity Plus¢ç 5' 6-FAM¢â / 3' IB¢çFQ250nm-·Î±×ÀÎ
51276250 nm Affinity Plus¢ç 5' Cy3¢â / 3' BHQ¢ç-2250nm-·Î±×ÀÎ
51277250 nm Affinity Plus¢ç 5' Cy3¢â / 3' IB¢çRQ250nm-·Î±×ÀÎ
51278250 nm Affinity Plus¢ç 5' Cy5 / 3' IBRQ250nm-·Î±×ÀÎ
51279250 nm Affinity Plus¢ç 5' Cy5¢â / 3' BHQ¢ç-2250nm-·Î±×ÀÎ
51280250 nm Affinity Plus¢ç 5' HEX¢â / 3' BHQ¢ç-1250nm-·Î±×ÀÎ
51281250 nm Affinity Plus¢ç 5' HEX¢â / 3' IB¢çFQ250nm-·Î±×ÀÎ
51282250 nm Affinity Plus¢ç 5' TEX¢â 615 / 3' BHQ¢ç-2250nm-·Î±×ÀÎ
51283250 nm Affinity Plus¢ç 5' TEX¢â 615 / 3' IB¢çRQ250nm-·Î±×ÀÎ
51284250 nm Affinity Plus¢ç 5' TYE¢â 563 / 3' BHQ¢ç-2250nm-·Î±×ÀÎ
51285250 nm Affinity Plus¢ç 5' TYE¢â 563 / 3' IB¢çRQ250nm-·Î±×ÀÎ
51286250 nm Affinity Plus¢ç 5' TYE¢â 665 / 3' BHQ¢ç-2250nm-·Î±×ÀÎ
51287250 nm Affinity Plus¢ç 5' TYE¢â 665 / 3' IB¢çRQ250nm-·Î±×ÀÎ
51288250 nm Affinity Plus¢ç 5' YAK¢ç / 3' IBFQ250nm-·Î±×ÀÎ
51346Affinity Plus¢ç Mini Probe 5' 6-FAM¢â / 3' IB¢çFQMini Probe-·Î±×ÀÎ
51347Affinity Plus¢ç Mini Probe 5' HEX¢â/ 3' IB¢çFQMini Probe-·Î±×ÀÎ
51348Affinity Plus¢ç Mini Probe 5' YAK¢ç / 3' IB¢çFQMini Probe-·Î±×ÀÎ
AP-100Affinity Plus Base (DNA & RNA )100 nmole100 nmole-·Î±×ÀÎ
AP-250Affinity Plus Base (DNA & RNA )250 nmole250 nmole-·Î±×ÀÎ
AP-1UAffinity Plus Base (DNA & RNA )1 umole1um-·Î±×ÀÎ
AP-5UAffinity Plus Base (DNA & RNA )5 umole5um-·Î±×ÀÎ
AP-10UAffinity Plus Base (DNA & RNA )10 umole10um-·Î±×ÀÎ

Affinity Plus qPCR Probes

Increase SNP target specificity and use shorter sequences with these high Tm probes

Use Affinity Plus qPCR Probes for SNP genotyping, transcript variant identification, and more sensitive target detection in challenging samples (FFPE tissue, biofluids). The Affinity Plus bases used in these qPCR probes include up to 6 locked nucleic acid monomers (see the technology page, Locked nucleic acids, for more information). When incorporated into a probe, they impart heightened structural stability, leading to increased hybridization melt temperature (Tm).

  • Improve mismatch discrimination compared to traditional qPCR probes
  • Benefit from this affordable approach to increasing assay hybridization specificity
  • Make flexible Tm adjustments more easily than possible with MGB probes

Ordering

Mini Affinity Plus qPCR Probes

Mini Affinity Plus qPCR Probes are ideal for screening small sample sets or performing just a few reactions when optimizing probe designs.

5' Reporter Dye(s) Quencher(s) Delivery Amount
0.5 nmol
FAM Iowa Black FQ *
HEX Iowa Black FQ *
YAK Iowa Black FQ *

Affinity Plus qPCR Probes

Affinity Plus qPCR Probes are offered with a wider selection of dyes and quenchers than Affinity Plus Mini qPCR Probes, and are best-suited for large-scale or high-throughput applications.

5' Reporter Dye Quencher Synthesis Scale Min Guarantee
5' 6-FAM¢â 3' Black Hole Quencher¢ç 1 1 µmole 20 nmoles
250 nmole 8 nmoles
3' Iowa Black¢ç FQ 1 µmole 20 nmoles
250 nmole 8 nmoles

5' Reporter Dye Quencher Synthesis Scale Min Guarantee
5' Cy3¢â 3' Black Hole Quencher¢ç 2 1 µmole 20 nmoles
250 nmole 8 nmoles
3' Iowa Black¢ç RQ-Sp 1 µmole 20 nmoles
250 nmole 8 nmoles

5' Reporter Dye Quencher Synthesis Scale Min Guarantee
5' Cy5¢â 3' Black Hole Quencher¢ç 2 1 µmole 20 nmoles
250 nmole 8 nmoles
3' Iowa Black¢ç RQ-Sp 1 µmole 20 nmoles
250 nmole 8 nmoles

5' Reporter Dye Quencher Synthesis Scale Min Guarantee
5' TEX 615 3' Black Hole Quencher¢ç 2 1 µmole 20 nmoles
250 nmole 8 nmoles
3' Iowa Black¢ç RQ-Sp 1 µmole 20 nmoles
250 nmole 8 nmoles

5' Reporter Dye Quencher Synthesis Scale Min Guarantee
5' TYE¢â 563 3' Black Hole Quencher¢ç 2 1 µmole 20 nmoles
250 nmole 8 nmoles
3' Iowa Black¢ç RQ-Sp 1 µmole 20 nmoles
250 nmole 8 nmoles

5' Reporter Dye Quencher Synthesis Scale Min Guarantee
5' TYE¢â 665 3' Black Hole Quencher¢ç 2 1 µmole 20 nmoles
250 nmole 8 nmoles
3' Iowa Black¢ç RQ-Sp 1 µmole 20 nmoles
250 nmole 8 nmoles

5' Reporter Dye Quencher Synthesis Scale Min Guarantee
5' HEX¢â 3' Black Hole Quencher¢ç 1 1 µmole 20 nmoles
250 nmole 8 nmoles
3' Iowa Black¢ç FQ 1 µmole 20 nmoles
250 nmole 8 nmoles

5' Reporter Dye Quencher Synthesis Scale Min Guarantee
5' Yakima Yellow¢ç 3' Iowa Black¢ç FQ 1 µmole 20 nmoles
250 nmole 8 nmoles
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