IDT
IDT
TransIT®-LT1 Transfection ReagentA broad spectrum, low toxicity, DNA transfection reagent
특징
TransITⓇ-LT1은 광범위한 세포에 고효율로 DNA를 transfection하는 편리한 제품입니다. 히스톤과 양이온 지질로 이루어진 시약으로 세포 독성이 낮은 것이 가장 큰 장점이며, 혈청의 유무에 상관없이 사용 가능합니다.  Figure 1. High Efficiency Transfection of iCell® Cardiomyocytes using TransIT®-LT1 Transfection Reagent. iCell Cardiomyocytes were plated at 20,000 cells/well in a 96 well tissue culture plate coated with 0.1% gelatin. After allowing the cells to recover from thaw, cells were transfected with 100 ng/well of pMAXGFP (Lonza) using TransIT®-LT1 Transfection Reagent with a 2:1 reagent-to-DNA ratio according to the manufacturer’s instructions. Fluorescent images were taken 3 days post transfection using a Olympus IX71® inverted microscope. See more information on stem cell applications.
 Figure 2. The TransIT®-LT1 Reagent Exhibits Higher Expression and Lower Cellular Toxicity Compared to Other Transfection Reagents. HepG2 cells were transfected with a luciferase expression plasmid using the designated reagents at the manufacturer’s recommended reagent-to-DNA ratio indicated beneath each bar. Transfections were performed in 96-well plates using 0.1 µg of plasmid DNA per well. Luciferase expression (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24 hours post-transfection. LDH levels are reported as % cytotoxicity compared to cells alone and were measured using a commercially available colorimetric assay; all values at or below zero are represented as zero on graph. Experiments were performed as per industry accepted testing protocols. FuGENE is a registered trademark of Fugent LLC. Lipofectamine is a trademark of Life Technologies Corporation.
 Figure 3. Comparable Luciferase Expression with the TransIT®-LT1 Reagent and FuGENE® 6 in Multiple Cell Types. The indicated cell lines were transfected in duplicate with 1 µg of a luciferase expression vector per well of a 12-well plate using either 3 µl of the TransIT®-LT1 or FuGENE® 6 Reagents according to industry accepted testing protocols. Cells were harvested 24 hours post-transfection and assayed for luciferase activity. FuGENE is a registered trademark of Fugent LLC.
 Figure 4. The TransIT®-LT1 Reagent Efficiently Delivers DNA to a Wide Variety of Cell Lines. Using the TransIT®-LT1 Transfection Reagent, cells were transfected with the pEGFP-C1 expression vector, and the percentage of EGFP expressing cells was determined 24-48 hours post-transfection.
Storage Conditions: Store at 4°C
Product Guarantee: 1 year ml001_transit_lt1_transfection_reagent.pdf (220,802kb) |
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