MyTaq¢â HS DNA Polymerase
CAT# PRODUCT SIZE STORAGE ¼ÒºñÀÚ°¡ ÁÖ¹®
BIO-21112MyTaq HS DNA Polymerase1000 UNITS-20-·Î±×ÀÎ
BIO-21113MyTaq HS DNA Polymerase2500 UNITS-20-·Î±×ÀÎ
-·Î±×ÀÎ
"The MyTaq HS sample was so much better than anything else we have used, we have ordered more already."

- Roslin Institute, Scotland

Features


• »õ·Ó°Ô °³¹ßµÈ antibody ±â¹ÝÀÇ hot-start polymerase
• ³ôÀº specificity ¿Í ¿ì¼öÇÑ performance
• »õ·Î¿î buffer : ultra-pure dNTPs ¿Í MgCl2
• ¹Ù·Î gel loadingÀÌ °¡´ÉÇÏ°Ô red-dye Æ÷ÇÔ °¡´É
• °£ÆíÇÑ all-in-one mastermix
 

Applications


High-throughput PCR
Assays with prolonged reaction setup on the bench or liquid handling
Amplification of challenging targets susceptible to mispriming
Amplification from challenging plant samples (second App Note)
Colony PCR
Multiplexing
Specific amplification of difficult templates (GC rich)
Genotyping
TA cloning
Two-Step RT-PCR

Description

MyTaqTM H S´Â c omplex g enomic D NA¿Í °° Àº ¾î·Á¿î template¸¦º¸´Ù ½±°í Á¤È®ÇÏ°Ô ÁõÆøÇϱâ À§ÇØ Bioline¿¡¼­ »õ·Ó°Ô °³¹ßÇÑ antibody ±â¹ÝÀÇ hot start PCR enzyme ÀÔ´Ï´Ù. ¼öÀ², °¨µµ, ¼Óµµ¸¦ ÁÁ°Ô ÇÒ ¼ö ÀÖµµ·Ï ÃֽŠ±â¼úÀ» ÀÌ¿ëÇÏ¿© PCR enzyme°ú DNA¿ÍÀÇ Ä£È­·ÂÀ» ³ô¿©ÁÖ¾ú½À´Ï´Ù. »ó¿Â¿¡¼­ reaction mix¸¦¸¸µé ¼ö ÀÖÀ¸¹Ç·Î ºñƯÀÌÀûÀÎ ÁõÆø°ú primer-dimer Çü¼ºÀ» ÇÇÇÒ ¼ö ÀÖ½À´Ï´Ù.
¶ÇÇÑ ÀÌ Á¦Ç°ÀÇ buffer¿¡´Â dNTPs, MgCl2, enhancerµéÀÌ ÃÖÀûÀÇ ³óµµ·Î µé¾î°¡ À־ optimization ¾øÀ̵µ ¿ì¼öÇÑ °á°ú¸¦ ¾òÀ» ¼ö ÀÖ½À´Ï´Ù. MyTaqTM HS Mix´Â ready-to-use 2x mix typeÀ¸·Î ºü¸¥ °á°ú¸¦ °¡Á®¿Ã ¼ö ÀÖ´Â °í°¨µµÀÇ PCR enzymeÀÔ´Ï´Ù. ÀÌ Á¦Ç°Àº ºü¸¥ cycling conditionÀ¸·Î ÁøÇà ÇÒ ¼ö ÀÖµµ·Ï setting µÇ¾î °¨µµ³ª ¼öÀ²Àº ³ôÀ¸¸é¼­ ½ÇÇè ½Ã°£Àº ´ÜÃà ½Ãų ¼ö ÀÖ½À´Ï´Ù. ¶ÇÇÑ MyTaqTM HS¿¡´Â red dye¸¦ Ãß°¡·Î Æ÷ÇÔÇÒ ¼ö ÀÖ½À´Ï´Ù. ÀÌ °æ¿ì¿¡´Â PCR ½ÇÇèÀÌ ³¡³ª°í È®ÀÎ ÀÛ¾÷ ½Ã µû·Î sample buffer¸¦ ³Ö¾îÁÙ ÇÊ¿ä ¾øÀÌ ¹Ù·Î agarose gel loadingÀÌ °¡´ÉÇÏ¿© º¸´Ù Æí¸®ÇÕ´Ï´Ù.

Components

MyTaq HS DNA Polymerase
5x MyTaq Reaction Buffer

Unit Definition

One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72¡ÆC.

Concentration

5u/¥ìl

Storage Conditions

MyTaq HS DNA Polymerase can be stored for up to 6 months at -20¡ÆC, or up to 2 weeks at +4¡ÆC. Repeated freeze/thaw cycles should be avoided.

Product Citations


Murray, S.A., et al. Appl. Envir. Microbiol. 77, 7050-7057(2011).

Notes

MyTaq is a trademark of Bioline.
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