SensiFAST¢â SYBR Lo-ROX One-Step Kit
CAT# PRODUCT SIZE STORAGE ¼ÒºñÀÚ°¡ ÁÖ¹®
BIO-74001SensiFAST SYBR Lo-Rox One-Step100 RXNSat -20C¡Æ-·Î±×ÀÎ
BIO-74005SensiFAST SYBR Lo-Rox One-Step500 RXNSat -20C¡Æ-·Î±×ÀÎ
"After getting nowhere with other kits we tried the SensiFAST SYBR One-Step Kit and saw results first time, I cannot describe how wonderful this made us feel."

 
- Asia-Pacific Special Nutrients Sdn. Bhd.
 

Features and Benefits


• ¹Î°¨¼º - ±¤¹üÀ§ÇÑ À¯ÀüÀÚ ¹ßÇöÀÇ Á¤È®ÇÑ Á¤·®
• ºü¸§ - ÃÖ°íÀÇ Æ¯À̼º°ú ¹Î°¨µµ¸¦ À§ÇØ µ¶Æ¯ÇÑ ¹öÆÛ »ç¿ë
• Bio-Rad ±â°è¿Í ȣȯ°¡´É - dynamic well factor¸¦ °è»êÇϱâ À§ÇØ flouresceinÀÌ ÇÊ¿äÇÑ ±â°è¿¡¼­ »ç¿ë(SensiFAST SYBR & Fluorescein kit)
• Æí¸®¼º - ºü¸£°í ½¬¿î one-tube ¼Â¾÷
• Protocol - fast qPCR °ú standard qPCR protocol·Î ¸ðµÎ »ç¿ë °¡´É
 

Instrument Compatibility


ABI 7500, 7500 FAST, Stratagene (Agilent) Mx4000¢â, Mx3000P¢â, Mx3005P¢â, as well as other instruments that do not require the use of a passive reference.

 

Description


SensiFAST SYBR One-step Kits´Â ºü¸£°í RT-qPCRÀ» À§ÇØ °³¹ßµÇ¾úÀ¸¸ç ÀϹÝÀûÀ¸·Î »ç¿ëµÇ°í ÀÖ´Â real-time ±â°è¿¡¼­ °ËÁõÀÌ µÇ¾ú½À´Ï´Ù. SensiFAST SYBR One-Step Kits´Â single tube¿¡¼­ ³ôÀº Àç»ý¼ºÀÇ first strand cDNA¿Í ÈÄ¼Ó real-time PCRÀÌ °¡´ÉÇϵµ·Ï Á¶¼ºµÇ¾ú½À´Ï´Ù. ÃÖ½ÅÀÇ buffer Á¶¼º°ú enhancer ±×¸®°í antibody ±â¹Ý hot-start DNA PolymeraseÀÇ Á¶ÇÕÀº SensiFAST SYBR kit°¡ ºü¸£°í ³ôÀº Àç»ýÀ²°ú ÃÖ°í·Î ¹Î°¨ÇÑ real-time PCRÀÌ °¡´ÉÇϵµ·Ï ÇÕ´Ï´Ù. ÀÌ kit´Â DNA template¿¡ ÀûÇÕÇÏ°í RNA templateÀº Bioline Tetro cDNA Synthesis Kit(BIO-65042)·Î revere transcriptionÇÑ ÈÄ »ç¿ë °¡´ÉÇÕ´Ï´Ù. SensiFAST One-step kit´Â 2X SensiFAST SYBR One-Step mix¿Í º°µµÀÇ reverse transcriptase, RiboSafe Inhibitor ·Î ±¸¼ºµÇ¾î ÀÖ½À´Ï´Ù. SensiFAST SYBR Hi-Rox kit´Â  ¼±Åûç¿ëÀ» À§ÇØ premixed ROX°¡ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù.
 

Volume


BIO-74001: 100 x 20µl Reactions: 1 x 1ml
BIO-74005: 500 x 20µl Reactions: 5 x 1ml

 

Components


2x Reaction mix consisting of: Heat-activated DNA polymerase Ultra-pure dNTPs MgCl2 ROX SYBR¢ç Green I
Plus separate vials of: Reverse transcriptase RNase inhibitor DEPC-treated water

 

Kit Size

The kit size is based on a 20µl final reaction volume.

 

Storage Conditions

12 months at -20¡ÆC. Avoid exposure of the SYBR¢ç Green I to light.

Notes


SensiFAST is a trademark of BiolineReagents Ltd.

»ç¿ë Èıâ
»ç¿ëÈıⰡ ¾ø½À´Ï´Ù.
»ç¿ëÈıâ ÀÛ¼º
°ü·ÃÁ¦Ç°
SensiFAST¢â SYBR Hi-ROX One-Step Kit
SensiFAST¢â SYBR No-ROX One-Step Kit
SensiFAST¢â SYBR & Fluorescein One-Step Kit
gototop