"After getting nowhere with other kits we tried the SensiFAST SYBR One-Step Kit and saw results first time, I cannot describe how wonderful this made us feel."
- Asia-Pacific Special Nutrients Sdn. Bhd.
Features and Benefits• ¹Î°¨¼º - ±¤¹üÀ§ÇÑ À¯ÀüÀÚ ¹ßÇöÀÇ Á¤È®ÇÑ Á¤·® • ºü¸§ - ÃÖ°íÀÇ Æ¯À̼º°ú ¹Î°¨µµ¸¦ À§ÇØ µ¶Æ¯ÇÑ ¹öÆÛ »ç¿ë • Bio-Rad ±â°è¿Í ȣȯ°¡´É - dynamic well factor¸¦ °è»êÇϱâ À§ÇØ flouresceinÀÌ ÇÊ¿äÇÑ ±â°è¿¡¼ »ç¿ë(SensiFAST SYBR & Fluorescein kit) • Æí¸®¼º - ºü¸£°í ½¬¿î one-tube ¼Â¾÷ • Protocol - fast qPCR °ú standard qPCR protocol·Î ¸ðµÎ »ç¿ë °¡´É Instrument CompatibilityRoche LightCycler¢ç 480 platform, Bio-Rad Opticon¢ç, Opticon¢â2, Chromo4¢â, MiniOpticon¢â, CFX96¢â, CFX384¢â, Cepheid SmartCycler¢ç, Qiagen Rotor-Gene¢â 3000, 6000, Eppendorf Mastercycler¢ç ep realplex and Techne Quantica¢ç. DescriptionSensiFAST SYBR One-step Kits´Â ºü¸£°í RT-qPCRÀ» À§ÇØ °³¹ßµÇ¾úÀ¸¸ç ÀϹÝÀûÀ¸·Î »ç¿ëµÇ°í ÀÖ´Â real-time ±â°è¿¡¼ °ËÁõÀÌ µÇ¾ú½À´Ï´Ù. SensiFAST SYBR One-Step Kits´Â single tube¿¡¼ ³ôÀº Àç»ý¼ºÀÇ first strand cDNA¿Í ÈÄ¼Ó real-time PCRÀÌ °¡´ÉÇϵµ·Ï Á¶¼ºµÇ¾ú½À´Ï´Ù. ÃÖ½ÅÀÇ buffer Á¶¼º°ú enhancer ±×¸®°í antibody ±â¹Ý hot-start DNA PolymeraseÀÇ Á¶ÇÕÀº SensiFAST SYBR kit°¡ ºü¸£°í ³ôÀº Àç»ýÀ²°ú ÃÖ°í·Î ¹Î°¨ÇÑ real-time PCRÀÌ °¡´ÉÇϵµ·Ï ÇÕ´Ï´Ù. ÀÌ kit´Â DNA template¿¡ ÀûÇÕÇÏ°í RNA templateÀº Bioline Tetro cDNA Synthesis Kit(BIO-65042)·Î revere transcriptionÇÑ ÈÄ »ç¿ë °¡´ÉÇÕ´Ï´Ù. SensiFAST One-step kit´Â 2X SensiFAST SYBR One-Step mix¿Í º°µµ reverse transcriptase, RiboSafe Inhibitor·Î ±¸¼ºµÇ¾î ÀÖ½À´Ï´Ù. SensiFAST SYBR Hi-Rox¿Í Lo-ROX One-Step kitÀº ¼±Åûç¿ëÀ» À§ÇØ premixed ROX°¡ Æ÷ÇÔ µÇ¾î ÀÖ½À´Ï´Ù. VolumeBIO-72001: 100 x 20µl Reactions: 1 x 1ml BIO-72005: 500 x 20µl Reactions: 5 x 1ml Components
2x Reaction mix consisting of:
Heat-activated DNA polymerase
Ultra-pure dNTPs
MgCl2
ROX
SYBR¢ç Green I
Plus separate vials of: Reverse transcriptase, RNase inhibitor, DEPC-treated water Kit SizeThe kit size is based on a 20µl final reaction volume. Storage Conditions12 months at -20¡ÆC. Avoid exposure of the SYBR¢ç Green I to light. NotesSensiFAST is a trademark of BiolineReagents Ltd. protocol [pi_50219_sensifast_sybr_lo_rox_one_step_kit_v1.pdf] (439,422kb) |
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