PrimeTime qPCR Primers (SYBR GreenÀ» ÀÌ¿ëÇÑ qPCR)SYBR Green qPCRÀ» À§ÇÑ Pre-designed Primers
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(500 reactions)
NCBI accession number¸¸À¸·Îµµ human, mouse, ratÀÇ transcriptomesÀÇ pre-designÀ» ¼±ÅÃÇÒ ¼ö ÀÖ½À´Ï´Ù. ¸ðµç PrimeTime qPCR Assays´Â Primer pair°¡ ÇÑ °³ÀÇ ´ÜÀÏ Æ©ºê¿¡ ´ã°Ü ¾à 3-4ÀÏ ¾È¿¡ Á¦Ç°À» ¼ö·ÉÇÒ ¼ö ÀÖ½À´Ï´Ù. ¸ðµç ¿Ã¸®°í´Â mass pectrometry¸¦ ÅëÇØ 100 % QC¸¦°ÅÄ£ ÈÄ¿¡ Á¦Ç°À¸·Î ÆÇ¸ÅµÇ¸ç ¸ðµç QC data´Â IDT ȨÆäÀÌÁö¿¡¼ ¾ðÁ¦µçÁö ¹«·á·Î ´Ù¿î·Îµå °¡´ÉÇÕ´Ï´Ù.
Primetime qPCR SYBR assay Ư¡••100 % QC oligos Á¦°ø
••Full sequences Á¦°ø
••90 %ÀÇ ¼º°øÀûÀÎ °á°ú º¸Àå
••Human, mouse, rat ÀÇ À¯ÀüÀÚÀÇ µðÀÚÀÎ
••Primer pair ¸¦ ÇÑ °³ÀÇ tube¿¡ ÇÔ²² Á¦°ø
••3-4ÀÏ ¼Ò¿ä ¿¹Á¤
PrimeTime¢ç qPCR Primer Assays °¡°Ý
Pre-designed PrimeTime¢ç qPCR Assay* 100 % °á°ú º¸Àå (positive¿Í negative control µ¥ÀÌÅÍ Á¦°ø ½Ã)
* Sequence Á¦°ø (Á¦Ç°°ú µ¿ºÀ)
* Human, mouse, rat ¼¼ °¡Áö species¿¡¼¸¸ µðÀÚÀÎ Á¦°ø
Pre-designed PrimeTime¢ç qPCR Assay´Â¾Æ·¡ ¸µÅ©¿¡¼ µðÀÚÀÎÀ» Á÷Á¢ ÇÒ ¼ö ÀÖ½À´Ï´Ù.http://sg.idtdna.com/order/predesignedassay.aspx?source=scitoolsSame High Efficiency With or Without Probe
Panel A. Amplification with SYBR Green Panel B. Amplification with Dual-Labeled Probe. Figure 1. PrimeTime¢ç Assays Yield the Same High Efficiency Whether Used With Intercalating Dyes or Probe. Amplification of 5 sequential 4-fold dilutions of cDNA using PrimeTime qPCR Primer Assays (with SYBR¢ç Green dye) or the PrimeTime qPCR 5' Nuclease Assay (with dual-labeled probe) to human 3-oxoacid CoA transferase 1 (OXCT1) (NM_000436). Figure 2. PrimeTime¢ç qPCR Primer Assays Have Average Reaction Efficiency >90%. Sixty randomly selected PrimeTime qPCR Primer Assays and 15 PrimeTime qPCR Primer Assays for endogenous control genes used with Brilliant III Ultra Fast SYBR¢ç Green qPCR Master Mix (Agilent) were analyzed over 5 sequential 4-fold dilutions (50–0.195 ng/reaction) of cDNA prepared from Universal Human Reference RNA (Agilent). Reactions were run on the 7900HT Fast Real-Time PCR System (Applied Biosystems) using PCR cycling conditions: 3 min 95¡ÆC; 45 x (5 sec 95¡ÆC, 15 sec 60¡ÆC). Average reaction efficiencies for the assays tested exceeded 98%. primetime-qpcr-primers-resuspension-protocol.pdf (151,611kb) primetime-qpcr-application-guide-3rd-ed-.pdf (8,682,050kb) |
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