"SensiMix products from Bioline are easy to use and give consistent results, they make the switch to real-time PCR very simple."
- Biobest Laboratories, The Edinburgh Technopole, Milton Bridge, Scotland.
Features and Benefits
Instrument Compatibility
Bio-Rad iCycler¢ç, iQTM5 and MyiQ¢â. (See product selection table). However the kit can is also compatible with several instruments that do not require the use of fluorescein, such as the Qiagen Rotor-Gene¢â 6000, the Bio-Rad CFX96 or the Roche LightCycler¢ç 480.
DescriptionThe SensiMix SYBR¢ç & Fluorescein Kit contains fluorescein, making it specifically suitable for use on real-time instruments that measure fluorescein signal, such as the Bio-Rad iCycler¢ç, iQ¢â5 and MyiQ¢â platforms. Both SYBR¢ç Green I and fluorescein are premixed for immediate use.
With SYBR¢ç Green I and fluorescein in their premixed and optimized concentrations, as few as 10 copies of a target gene can be detected. VolumeQT615-02: 250 x 50µl Reactions: 5 x 1.25ml QT615-05: 500 x 50µl Reactions: 10 x 1.25ml QT615-20: 2000 x 50µl Reactions: 40 x 1.25ml Components2x Reaction mix consisting of: Heat-activated DNA polymerase Ultra-pure dNTPs
MgCl2 (6mM)
Fluorescein SYBR¢ç Green I
Plus separate vials of: 50mM MgCl2 Kit SizeThe kit size is based on a 50µl final reaction volume. Storage Conditions
6 months at -20¡ÆC. Avoid exposure of the SYBR¢ç Green I to light.
Product CitationsDhawan R. et al. Plant Cell 21: 1000-1019 (2009). Maatouk D.M. et al. Hum. Mol. Genet. 17(19): 2949-2955 (2008). Del Rosario B.C. & Pemberton L.F. Mol. Cell. Biol. 28(7): 2113-2124 (2008). Miller M.R. et al. J. Nutr. 138(11):2179-2185 (2008). NotesSensiMix is a trademark of Bioline Reagents Ltd. protocol [pi_50230_sensimix_sybr_fluorescein_kit_v1.pdf] (433,480kb) |
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