LandMark¢â Mid Range Prestained Protein Marker(all blue)
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20031LandMark¢â Mid-Range Prestained Protein Marker(all blue)500ul-20¡ÆC-·Î±×ÀÎ

LandMark¢â Mid Range Prestained Protein Marker(all blue)

 

Description

LandMarkTMmid-range prestained protein molecular weight standard is a blue color prestained size marker. It is a mixture of 8 recombinant purified and prestained polypeptides whose molecular weights are well-adjusted. Prestained protein molecular weight standard is available in a ready-to-use formula (no need to boil before use), and is suitable for sizing proteins between 15,000 and 140,000 Da by SDS-PAGE. A blue chromophore is covalently bound to proteins, and 8 prestained proteins are visible during electrophoresis or electrophoretic transfer from the gel to a membrane. Because coupling of chromophore to the proteins affects their apparent molecular weights in SDS-PAGE, unstained protein standard should be used for accuracy >95%.

Features

  • Mid range size estimation  
  • Highly purified and blended 8 recombinant proteins
  • His-tag sequence in the proteins enables direct detection on western blots using anti-his antibody  
  • User oriented ready to use solution

Recommended storage condition

•  -20¡É for one year
• Please aliquot in a small volume for prevention of repeated freezing & thawing

Usage information

•  Recommended loading volume:
          5µl/lane for mini gel(10x8cm, 0.75 or 1mm thick)
          10-15µl/lane for large gel(30x20cm, 1 or 1.5mm thick)
•  Typical number of lanes: 100
•  Number of Bands: 8
•  Range (KDa): 15-140
 

20031.jpg 10% Gradient Gel, 5ul loading

 
20031.pdf (194,182kb)
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