°¡Àå ¸¹ÀÌ »ç¿ëµÇ´Â Mid Range Prestained Protein Maker ÀÔ´Ï´Ù. 50 kDa »çÀÌÁîÀÇ proteinÀÌ ÇÎÅ©»öÀ¸·Î ¿°»öµÇ¾î ÀÖ°í ³ª¸ÓÁö proteinµéÀº ÆĶõ»öÀ¸·Î ¿°»öµÇ¾î ÀÖ´Â dual color marker ÀÔ´Ï´Ù. SharpÇÏ°í Á¤È®ÇÑ recombinant proteinÀ¸·Î ´õ¿í Á¤È®ÇÑ ´Ü¹éÁúÀÇ »çÀÌÁ ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù.
DescriptionLandMarkTMmid range prestained protein marker is a dual color prestained size marker. It
is a mixture of 8 recombinant purified and prestained polypeptides whose
molecular weights are well-adjusted. It is available in a ready-to-use formula
(no need to boil before use), and is suitable for sizing proteins between
15,000 and 140,000 Da by SDS-PAGE. A blue and pink chromophore is covalently
bound to proteins, and 8 prestained proteins are visible during electrophoresis
or electrophoretic transfer from the gel to a membrane. The 50 KDa proteins
labeled with pink chromophore enable easy identification.
Because coupling of
chromophore to the proteins affects their apparent molecular weights in
SDS-PAGE, unstained protein standard should be used for accuracy >95%.
Features • Dual color size standard (blue and pink)
• Mid range size estimation
• Highly purified and blended 8 recombinant proteins
• His-tag sequence
in the proteins enables direct detection on western blots using anti-his
antibody
• User oriented ready to use solution
Recommended storage condition• -20¡É for one year
• Please
aliquot in a small volume for
prevention of repeated freezing & thawing
Usage information• Recommended loading volume:
5µl/lane for mini gel(10x8cm, 0.75 or 1mm thick)
10-15µl/lane for large gel(30x20cm, 1 or 1.5mm thick)
•Typical number of lanes: 100
•Number of Bands: 8
• Range (KDa): 15-140
12% SDS-PAGE Gel, 5ul loading data20030.pdf (171,522kb) |
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