IDT
IDT
Nuclease Detection ProductsIDT에서 개발한 RNases와 DNases detection Kit로 빠르고 민감하게 측정할 수 있습니다. Nuclease가 degradation될 때에만 형광을 나타나게 하는 원리로 쉽게 확인 할 수 있습니다. 또한 이 assay는 enzyme kinetics 연구에서 정량적으로 사용할 수 도 있습니다.
Nucleases are widely present in the laboratory environment and can interfere with many experiments. In particular, single-stranded RNases are ubiquitous, hard to eliminate, and can rapidly degrade important samples used in microarray studies, real-time PCR, northern blots, or cDNA cloning.
IDT has developed reagents that allow for rapid, sensitive detection of RNases and DNases. These reagents are fluorescence-quenched oligonucleotide probes that emit a fluorescence signal only after nuclease degradation. The assay can be read visually or measured and quantified using fluorometry. Assays can be used qualitatively to test lab reagents, equipment, and supplies for nuclease contamination. Assays can be used quantitatively to study enzyme kinetics. Two oligonucleotide substrates are available: one designed to detect RNases and the second designed to detect DNases. The RNaseAlert® substrate employs a FAM™ reporter (Em 520 nm). The DNaseAlert™ substrate employs a HEX™ reporter (Em 555 nm).
RNaseAlert and DNaseAlert are available as:
Single-use tubes (25 tubes with 0.05 nmole each)—ideal for rapid hand-held visual assays at the research bench
Bulk substrate (2 tubes with 2 nmole each)—for use in either visual assays or read in a fluorometer
Kit—includes single use tubes, buffer, water, enzyme, and nuclease decontamination solution
RNaseAlert® Kit Components:25 50 pmole Fluorescent Substrate tubes
250 µL RNaseAlert® Buffer
2 mL Nuclease-Free water
50 µL RNase A
50 mL Nuclease Decontamination Solution
Kit quality guaranteed for six months from date received when stored at –20°C.
DNaseAlert™Kit Components:25 50 pmole Fluorescent Substrate tubes
250 µL DNaseAlert™ Buffer
2 mL Nuclease-Free water
25 µL 2U/mL DNase 1 Enzyme
50 mL Nuclease Decontamination Solution
Kit quality guaranteed for six months from date received when stored at –20°C.
 DNaseAlertTM_User_Guide.pdf (141,414kb) dnasealert-user-manual.pdf (626,567kb) RNaseAlertTM_User_Guide.pdf (137,004kb) user-guide-rnasealert.pdf (623,521kb) |
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