Label IT¢ç RNAi Delivery ControlsFluorescently labeled RNAi duplexes ideal for in vitro or in vivo tracking
Figure 1. The Label IT¢ç RNAi Delivery Controls Allow Quick Assessment of Delivery Efficiency For In Vitro Applications. HeLa cells were transfected in serum-containing media with (A) Label IT¢ç Cy¢ç3 RNAi Delivery Control (red) using the TransIT-siQUEST¢ç Transfection Reagent and (B) Label IT¢ç Fluorescein RNAi Delivery Control (green) using the TransIT-TKO¢ç Transfection Reagent. Twenty-four hours post-transfection, the cells were fixed, then counterstained to locate the nuclei (blue) and the actin (green).
Figure 2. Visualization of the Label IT¢ç Cy¢ç3 RNAi Delivery Control in Liver Sections Following Tail Vein Injection. TransIT¢ç-QR Hydrodynamic Delivery Solution was used to deliver 25 µg of Label IT¢ç Cy¢ç3 RNAi Delivery Control (red) to a mouse using hydrodynamic delivery via the tail vein. Forty-five minutes post-injection the liver was harvested. Sections were processed to stain the nuclei (blue) and to stain the actin (green).
Storage Conditions: Store at -20¨¬C, protected from exposure to light. Store the 10X RNAi Dilution Buffer at 4¨¬C.
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