Electrophoresis system

 

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Quick Start Manual

After the agarose gel has solidified, sample loading and electrophoresis can begin.

Agarose gels can be run in many different types of electrophoresis buffers.

Nucleic acid agarose gel electrophoresis is usually conducted with either Tirs-Acetate-EDTA(TAE) buffer or Tris-Borate-EDTA(TBE) buffer. While TAE buffer provides faster electrophoretic migration of linear DNA and better resolution of supercoiled DNA, TBE buffers have a stronger buffering capacity for longer or higher voltage electrophoresis runs.

1. Prepare sampels for gel loading. Lading volume is dependent upon the type of comb used(i.e., well thickness and length) and thickness of the gel.

2. When lading volume is determined, add standard nucleic acid sample lading dye to a final 1X concentration to make samples dense for underlaying into sampel wells.

3. Load the samples into the wells using standard pipets.

4. Place the lid on the cell carefully. Do not distrub the samples. This system lids attach ot the base in only one orientation. To attach the lid correctly, match the red and black banana jacks on the lid with the red and black banana plugs of the base.

5. Power requirements very depending on gel thickness, length and concentration, and type of electorphoresis buffer used.