IDT
IDT
 α-Select Bronze Competent Cells are ideal for routine subcloning.
Features & BenefitsReduced recombination of cloned DNA - designed to accommodates larger plasmids
High efficiency - available in ≥107 cfu/μg, perfect for routine subcloning
Easy screening - with blue/white color selection
Convenient size - small aliquots reduce waste
ApplicationsTransformation of large plasmids into bacterial cellsGenerating clones Blue/white color screening
Descriptionα-Select Bronze Competent Cells are directly comparable to DH5α cells. They contain a lacZ marker that provides α-complementation of the ß-galactosidase gene for blue/white color screening and are ideal for subcloning using plasmid-derived vectors. α-Select Bronze Competent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of the plasmid DNA, allowing efficient transformation even with large plasmids.
pUC19 DNA is also provided as a positive control. NotesGenotype: F- deoR endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) supE44 thi-1 phoA Δ(lacZYA-argF)U169 Φ80lacZΔM15 λ-
Components
Storage & StabilityAll kit components should be stored at -80°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label. Shipping conditionsShipped on Dry Ice or Blue Ice. |
사용후기 작성
|
