Alpha-Select Gold Efficiency
CAT# PRODUCT SIZE STORAGE ¼ÒºñÀÚ°¡ ÁÖ¹®
BIO-85027Competent Cells, Gold Efficien20 X 50¥ìLat -80C¡Æ-·Î±×ÀÎ
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¥á-Select Gold Competent Cells are ideal for generating high efficiency libraries cloning with limited amounts of DNA.
 
 

  
 

Features & Benefits

Reduced recombination of cloned DNA - accommodates larger plasmids
High efficiency - available in ¡Ã109 cfu/¥ìg, perfect for library construction
Easy screening - with blue/white color selection
Convenient size - small aliquots reduce waste

Applications

Transformation of large plasmids into bacterial cells Generating cDNA libraries Blue/white color screening

Description

¥á-Select Gold Competent Cells are directly comparable to DH5¥á cells. They contain a lacZ marker that provides ¥á-complementation of the ©¬-galactosidase gene for blue/white color screening and are ideal for construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors.
¥á-Select Gold Competent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of the plasmid DNA, allowing efficient transformation even with large plasmids.
pUC19 DNA is also provided as a positive control.

Notes

Genotype: F- deoR endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) supE44 thi-1 phoA ¥Ä(lacZYA-argF)U169 ¥Õ80lacZ¥ÄM15 ¥ë-
 

Components

Reagent
BIO-85027
Chemically Competent Cells
20 x 50¥ìl
pUC19 control vector (10pg/¥ìl)
100¥ìl

Storage & Stability

All components should be stored at -80¡ÆC upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

Shipping conditions

Shipped on dry ice.
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