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NxSeq¢ç AmpFREE Low DNA Library Kits and Adaptors (Illumina-compatible)

 The highest efficiency NGS fragment library prep kit available at the lowest cost 

• Great Performance: High efficiency libraries mean more sequenceable fragments in each library, better coverage & depth from single or multiplexed libraries which improves confidence in each sequencing run.
• Minimal Bias: PCR-free DNA libraries produce more uniform coverage from as little as 75 ng of sheared input DNA.
• Fast: 2 hour, 10 minute protocol saves you time and gets your samples on the sequencer sooner.
• Affordable: Best priced and best performing kit available.

Highly Mappable Reads (>92%) from Human, Staphylococcus and Rhodobacter gDNA Sequencing

Sequencing Stat	Human	Staphylococcus	Rhodobacter
Genome size, GC percentage	~3 Gbp  45% GC	2,821,361  33% GC	4,602,977  69% GC
Raw reads	3,131,114	1,260,836	3,900,174
Mapped reads	2,979,237 (95.15%)	1,174,111 (93.12%)	3,613,165 (92.64%)
Read length	148.9 bp	148.8 bp	149.6 bp
Total bases	443,767,447	174,694,261	540,403,552
Genome fraction	0.11	0.97	1.00
Avg. coverage	0.15X	62X	117X
Figure 3. Representative gDNA sequencing stats from three different organisms. Genomic DNA fragment libraries were generated using the NxSeq AmpFREE Low DNA Library Kit using 75 ng of sheared gDNA input from three organisms (human, Staphylococcus aureus, and Rhodobacter sphaeroides). The final libraries were quantitated and normalized to 2 nM final concentrations using the Bioanalyzer and Qubit fluorometer, and 5 µL of each library was run on a MiSeq using 2 x 150 bp chemistry and analyzed. Minimal Bias Detected   Figure 4. Sequencing bias measured for three different organisms with varying GC content. DNA fragment libraries were generated from gDNA of three organisms with varying GC content (Staphylococcus aureus, 24% GC; E. coli K12, 50% GC; and Rhodobacter sphaeroides, 68% GC) according to the manufacturer¡¯s recommended input amounts and protocols. Samples were quantitated using the Bioanalyzer and Qubit fluorometer  and normalized to 2 nM final concentrations. Five µL of each library sample was sequenced on a MiSeq using 2 x 150 bp v2 chemistry and analyzed. Normalized coverage was calculated as the (average coverage of all windows with X% GC content) divided by the (overall average coverage). Faster Protocol with Significantly Less Hands-on Time