ExAct^TMPlus Taq DNA Polymerase is an improved form of ExAct^TMTaq DNA Polymerase and can amplify target DNA at a broad-range of annealing temperature. Its unique buffer system reduces primer-dimer formation and non-specific amplification. ExAct^TM Plus Taq DNA Polymerase possesses the greater yield, processivity and fidelity than normal Taq polymerase. The fidelity of ExAct^TMPlus Taq DNA Polymerase is higher approximately 4 times than that of Taq polymerase. In case of PCR amplification of target DNA with high G+C content or structural problem, such as repeat sequence, the application of 5X Q Plus Buffer(optional) improves the specificity and productivity of the reaction.

• PCR amplification of long DNA fragments up to 10 kb

• Extreme productivity

• High specificity and sensitivity

• RT-PCR

• Generation of PCR products for TA cloning

 

• Higher fidelity than standard Taq DNA polymerase

• Effectively amplifies problematic, complex or GC-rich templates

• Chemical Hot Start Taq DNA polymerase

• Long PCR up to 10kb

• Broad range annealing temperature

 

 

 

PCR Reaction Conditions
¡¡	25 ul volume	50 ul volume
Template	X ul	X ul
Forward Primer (10 pmoles/¥ìl)	1 ul	2 ul
Reverse Primer (10 pmoles/¥ìl)	1 ul	2 ul
5x Reaction Mix [with dNTPs]	5 ul	10 ul
5x Q Plus Buffer(optional)	0 ~ 10 ul	0 ~ 20 ul
ExActTM Plus	1 ~ 1.25 units	1 ~ 1.25 units
Taq DNA Polymerase
Distilled water	up to 25 ul	up to 50 ul

¡Ø 5x Q Plus Buffer is an additive altering the binding behavior of primer and template and can help the amplification that do not work well under standard PCR condition. Especially, 5x Q Plus Buffer can be used for the amplification of problematic template, such as high G+C content and repeat sequence regions. 5x Q Plus Buffer uses as adding into PCR reaction mixture from 0.5x to 2x.

 

 

 

Human genomic DNA	10 ng – 100 ng
Bacterial genomic DNA	5 ng – 50 ng
Purified plasmid or phage DNA	1 ng – 5 ng

 

 

50% Glycerol, 20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20, 0.5% Nonidet P-40, 1 mM PMSF

The ExAct^TM Plus Taq DNA Polymerase is shipped on Dry/Blue Ice. All kit components should be stored at -20¡É upon receipt. Excessive freeze/thawing is not recommended. When stored under optimum conditions, the reagents are stable for a minimum of 6 months from date of purchase.

ExAct^TMPlus Taq DNA Polymerase was passed from quality control assay for contamination of endo- or exodeoxyribonuclease and the bacterial host DNA host.

Harmful if swallowed. Irritating to eyes, respiratory system and skin. Please refer to the material safety data sheet for further information.

1u is defined as amount of enzyme that required to catalyze the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 72¡ÆC.

• Recombinant ExAct^TM Plus Taq DNA Polymerase is the enzyme of choice for most PCR applications.

• The number of PCR cycles depends on the amount of template DNA in the reaction mix and on the expected yield of the PCR product. 25 -35 cycles are usually sufficient for the majority PCR reaction. Low amounts of starting template may require 40 cycles.