Mbiotech Agarose

 

 

 

Agarose is a neutral polysaccharide extracted from certain seaweeds.  The molecular structure of this polysaccharide gives the capacity to form gels that are very strong even at low concentrations.  Agarose is the most popular medium for electrophoresis. Mbiotech's agaroses are reliable and high-quality products.

 

‧ For DNA/RNA routine analysis, 100bp - 25 kb

‧ Low EEO for high electrophoresis mobility

‧ High purity and Multi-purpose

‧ Greener agarose choice

‧ Low background and high resolution

‧ Optimized gel strength for ease of gel preparation

‧ Ideal for gel electrophoresis, chromatographic separations, and other life science applications.

 

1. Gel Electrophoresis

2. Gel plates

3. Gel matrix used in chromatographic separations

4. IEP (Immuno electrophoresis) and Ouchterlony (double diffusion) plates in which antibody-antigen precipitin lines are studied

 

 

         1% Agarose Gel

 

        2% Agarose Gel

 

        3% Agarose Gel

 

 

 

 

Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture. 
Gelling point - the temperature at which an aqueous agarose solution forms a gel as it cools.
Electroendosmosis (EEO) - one of the most important characteristics of agarose. Anionic group in agarose gel are affixed to the matrix and can not move, but dissociable counter cations can migrate towards the cathode in the matrix, giving rise to EEO, EEO can disrupt separations of DNA because of internal convection. The lower EEO, the faster DNA will migrate, additionally, lower EEO helps to improve the resolution of DNA and RNA as their migration is determined only by their size, not by their charge.