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READY TO USE BUFFERS PROVIDE CONVENIENCE AND REPRODUCIBILITY
Mbiotech offers high quality running buffers for all applications; both nucleic acid and protein. Premixed electrophoresis buffers eliminate variables which can distort data and give unreliable results. Premixed buffers are made from the high quality reagents and are carefully mixed and tested to ensure reproducibility. Ten liters of 1X buffer can be made from each bottle of 10X stock. Tris-Glycine-SDS Running Buffer (10X)
Premixed 10 X gel Running buffer for SDS-PAGE
0.25M Tris(pH 8.6)
1.92M Glycine 1% SDS
Tris-Glycine Runnung Buffer(10x)
Premixed 10 X gel Running buffer for a native PAGE(1 Liter,10 X) 0.25 M Tris(~ pH 8.6)
1.92M Glycine
Tris-Tricine-SDS Running Buffer(10 X)
Premixed 10 X gel Running buffer for a peptide SDS-PAGE(1Liter, 10X)
1M Tris(~pH 8.3)
1M Tricine
1% SDS
1M Tris-HCl(pH 6.8)
1.5M Tris-HCl(pH8.8)
TBE Buffer(10 X)
Premixed 10 X Tris-borate-EDTA buffer for nucleic acid electrophoresis (1Liter, 10X)
0.89M Tris-borate, pH 8.3, 0.02M EDTA
TAE Buffer (50 X)
Premixed 50 X Tris-acetate-EDTA buffer for nucleic acid electrophoresis(1 Liter, 50 X)
2M Tris-acetate, 50mM EDTA
Protein Extraction Solution (RIPA)
150 mM Sodium chloride, 1% Trition X-100, 1% Sodium deoxycholate, 0.1% SDS, 50mM Tris-HCl, pH7.5 and 2mM EDTA, sterile solution
Protein Extraction Solution (NP-40)
10% Solution of NP-40 in water
10X TBS Buffer
247mM Tris,27mM potassium Chloride, 1.37M Sodium Chloride, pH7.4,
sterile Solution
Sample Buffer, Laemmli(2X)
Tris-HCl pH 6.8 0.125M, 2% SDS ,2% ¥â-Mercaptoethanol, Glycerol 20%(w/v),
0.002% Bromophoenol Blue
Sample Buffer, Laemmmli(5X)
Tris-HCl pH 6.8 0.625M, 10% SDS, 2% ¥â-mercaptoethanol, Glycerol 30%(w/v),
0.1% Bromophoenol Blue
20X PBS(pH 7.4)
2.7M Sodium chloride, 54mM Potassium chloride, 86mM sodium phosphate (diabasic, anhydrous),
28mM potassium phosphate(monobasic anhydrous), pH 7.2 when diluted to 1 X, sterile solution.
50X TAE Buffer
2M Tris-Acetate, 50mM EDTA
10 X TE Buffer(pH 8.0)
100mM Tris-HCl, pH 8.0, 10mM EDTA, pH 8.0
Ethidium Bromide Solution
10mg/ml in distilled water
6X DNA Loading Buffer
0.25% Bromophenol blue, 0.25% Xylene cyanol FF, 30% Glycerol
500mM EDTA, pH 8.0
500mM Na2EDTA, pH adjusted to 8.0 with sodium hydroxide
Water-DEPC Treated
Purified by ion exchange, carbon extraction, glass distillation,
and filtration, Resistance greater than 18.0 M ohms, Treated
with 0.1% DEPC(diethypyrocarbonate)
Loading buffer for Native-PAGE, 4X
Loading buffer for SDS-PAGE, 5X
250mM Tris-HCl, pH 6.8, 0.5 M DTT,10% SDS
0.5% bromophenol blue, 50% Glycerol
1M Tris-HCl, pH 7.4
1M Tris(hydroxymethyl) aminomethane buffer,
pH 7.4. pH adjusted with hydrochloric acid, sterile solution
1M Tris-HCl, pH 8.5
1M Tris(hydroxymethyl) aminomethane buffer,
pH 8.5. pH adjusted with hydrochloric acid, sterile solution
1M Tris-HCl, pH 8.8
1M Tris(hydroxymethyl) aminomethane buffer,
pH 8.8. pH adjusted with hydrochloric acid, sterile solution
0.5M Tris-HCl, pH 6.8
0.5M Tris(hydroxymethyl) aminomethane buffer,
pH 6.8. pH adjusted with hydrochloric acid, sterile solution
1M Tris-HCl, pH 9.0
1M Tris(hydroxymethyl) aminomethane buffer,
pH 9.0. pH adjusted with hydrochloric acid, sterile solution
Ammonium Acetate Solution 7.5M
7.5M ammonium acetate, sterile solution
Ammonium Acetate Solution 10M
10M ammonium acetate, sterile solution
10% Ammonium Persulfate Solution
1M DTT Stock Solution
1 M DTT, 0.01 M Sodium acetate, pH 5.2, sterile solution
50% Glycerol Solution
50% Glycerol Solturion
1M HEPES Stock Solution
1M IPTG Stock Solution
1M IPTG, sterile filtered
X-gel Solution(40mg/ml)
40mg X-gel / 1ml DMF
10% Triton X-100 Solution
10% solution of Triton X-100 water. |
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