BradfordRED Kit

BradfordRED Kit

BradfordRED Kit.jpg
 
Expedeon's BradfordRed is a quick and ready-to-use colorimetric protein assay with increased linearity and increased detergent- and reducing agent-compatibility for convenient protein quantitation at 660nm
 
Expedeon's BradfordRed is a quick and ready-to-use colorimetric protein assay with increased linearity and increased detergent- and reducing agent-compatibility for convenient protein quantitation at 660nm

BradfordRed
Detergent compatible protein quantitation

Expedeons BradfordRed has a greater linear range than Coomassie-based Bradford assays. It is compatible with higher concentrations of most detergents (up to 5% for some detergents, e.g. Brij and CHAPS), reducing agents and other commonly used reagents. The accessory ionic detergent compatibility reagent (IDCR) creates an even broader detergent compatibility. BradfordRed is suitable for samples containing Laemmli SDS sample buffer with bromophenol blue.

Benefits:

  • Enhanced linearity
  • Greater range and concentration of detergent compatibility
  • Reducing agent compatible
  • Only 10ul sample required
  • Fast
  • Convenient room temperature storage
BradfordRed produces significantly less protein-to-protein variation compared to competitor Bradford assays, although it produces more protein-to-protein variability than BradfordMx. Please note that appropriate standards should be used due to the inherent protein variability of all protein assays. The BradfordRed assay can be performed in either a test tube or microplate format.
Bradford Red assay
Standard curves for BSA using BradfordRed and competitor B Bradford assay. Absorbances were measured at 660nm for the BradfordRed assay and 595nm for the competitor Bradford assay. BradfordRed has a greater linear range (25 to 2000µg) than the competitor Bradford assay (125 to 1000µg). 
BradfordRED Protein Quantitation
BradfordRed is based on the binding of a dye-metal complex to protein in acidic conditions that causes a shift in the dye's absorption maximum, which is measured at 660nm. The colour produced is stable and increases in proportion to a broad range of increasing protein concentrations, even in the presence of detergents and reducing agents that would be incompatible with standard Bradford and BCA protein assays. The ionic detergent compatibility (IDCR) reagent may be added to the assay reagent to increase compatibility with high amounts of ionic detergents, to measure samples containing Laemmli SDS sample buffer with bromophenol blue.
 
BradfordRed
Detergent compatible protein quantitation

Expedeons BradfordRed has a greater linear range than Coomassie-based Bradford assays. It is compatible with higher concentrations of most detergents (up to 5% for some detergents, e.g. Brij and CHAPS), reducing agents and other commonly used reagents. The accessory ionic detergent compatibility reagent (IDCR) creates an even broader detergent compatibility. BradfordRed is suitable for samples containing Laemmli SDS sample buffer with bromophenol blue.

Benefits:

  • Enhanced linearity
  • Greater range and concentration of detergent compatibility
  • Reducing agent compatible
  • Only 10ul sample required
  • Fast
  • Convenient room temperature storage
BradfordRed produces significantly less protein-to-protein variation compared to competitor Bradford assays, although it produces more protein-to-protein variability than BradfordMx. Please note that appropriate standards should be used due to the inherent protein variability of all protein assays. The BradfordRed assay can be performed in either a test tube or microplate format.
Bradford Red assay
Standard curves for BSA using BradfordRed and competitor B Bradford assay. Absorbances were measured at 660nm for the BradfordRed assay and 595nm for the competitor Bradford assay. BradfordRed has a greater linear range (25 to 2000µg) than the competitor Bradford assay (125 to 1000µg). 
BradfordRED Protein Quantitation
BradfordRed is based on the binding of a dye-metal complex to protein in acidic conditions that causes a shift in the dye's absorption maximum, which is measured at 660nm. The colour produced is stable and increases in proportion to a broad range of increasing protein concentrations, even in the presence of detergents and reducing agents that would be incompatible with standard Bradford and BCA protein assays. The ionic detergent compatibility (IDCR) reagent may be added to the assay reagent to increase compatibility with high amounts of ionic detergents, to measure samples containing Laemmli SDS sample buffer with bromophenol blue.
 
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