Nickel-NTA

Amintra NI-NTA resin

 
Amintra Affinity resins are part of a range of cost-effective chromatography resins designed for superior purification performance and customer satisfaction. Amintra resins benefit from high binding capacity, excellent specificity & resolution. They have outstanding durability & stability are high flow rate compatible and suitable for all types of chromatography. Amintra resins are available in prepacked columns (1 and 5ml) and as a slurry.
Amintra NI-NTA resin.jpg
 
 
 
Expedeon's Ni-NTA affinity resin is designed for simple, rapid His-tagged recombinant protein purification from a cell lysate under native or denaturing conditions. Amintra Ni-NTA offers excellent flow properties and high protein binding capacity: in excess of 50mg protein/ml.
NTA is a tetradentate chelator which occupies four of the six binding sites in the coordination sphere of the nickel ion. The other two coordination sites are usually occupied by water molecules and can interact with histidine residues of the recombinant protein. This binding minimizes metal leaching during purification.
Benfits of Ni-NTA
  • One step purification
  • High capacity
  • Purification under native or denaturing conditions
  • Minimum metal leaching
Specifications
Supporting matrix Covalently coupled to agarose resin
Charged metal ion
Ni2+
Bead size range 45-165 m
Recommended working pH pH 2.0-12.0
Max binding capacity 50 mg His-tagged recombinant protein/ml resin
Linear Flow rate Up to 300 cm/h (5cm diameter column, pressure 1 bar)
Optimum Flow Rate 1-10 ml/min
Maximum pressure 0.1MPa (1 bar)
Chemical stability High
Solubility in water Insoluble
 
 
 
 
 
 
 
 
 
 
 
 
 
Clarified E. coli lysate (10 ml) was purified on a 1ml Amintra NiNTA column (2,5 cm). Binding buffer: 50 mM Na2HPO4, 300 mM NaCl, 10 mM Imidazole, pH 8.0. Elution buffer: 50 mM Na2HPO4, 300 mM NaCl, 250 mM Imidazole, pH 8.0. Flow Rate: 1 ml /min.
 
 
Chemical Compatibility


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