Amintra Nickel Magnetic

Magnetic Resin His Tagged

His-tags have been widely employed as a powerful separation approach in the purification of a broad range of proteins and peptides. It is based on the specific interactions between certain transitional metal ions, mostly Cu2+, Ni2+, Zn2+ and Co2+ to the exposed amino acid surface chains containing histidine. The presence of several adjacent histidines such as (His)6-tag increases the affinity to immobilised metal ions. Increasingly, Amintra Ni-NTA and Ni-IDA resins are employed for the purification of histidine-tagged recombinant proteins expressed in bacteria, yeast and mammalian cells.

Amintra Nickel Magnetic resin is typically useful in purifying small quantity low concentration histidine-tagged proteins. For example, when 10 – 50 µl of resin is required for protein purification, it is difficult to handle such small quantities in columns. Amintra Nickel Magnetic resins can be easily processed with the aid of a magnet. The liquid / solid separation required in each purification step (e.g. equilibration, binding, washing and elution) is readily achieved with the aid of a magnet (e.g. magnetic stirring bar or other commercial magnetic devices).

Key benefits:

Tailored base matrix design for batch magnetic purification of proteins
High binding capacity (could be over 40 mg/ml)
Simple and convenient operation, no chromatography training required
Flexible operational conditions
Low cost

Characteristics of Amintra Nickel Magnetic

Particle size	50 – 150 µm
Base matrix	Cross-linked 6% agarose encapsulating magnetic particles
Metal ion capacity	Approx. 12 - 25 µmol / ml resin
Binding capacity	Up to 40 mg/ml resin*
Chemical stability	Stable in 0.01M HCl and 1% SDS tested for 30 mins; 0.5 M NaOH tested for overnight;
pH stability	2-14 (<2 h) 4-12 (up to one week)
Storage	20% ethanol at 4^oC
Supplied as	50% Slurry