Ni-Magnetic

Amintra Nickel Magnetic

Magnetic Resin His Tagged

His-tags have been widely employed as a powerful separation approach in the purification of a broad range of proteins and peptides. It is based on the specific interactions between certain transitional metal ions, mostly Cu2+, Ni2+, Zn2+ and Co2+ to the exposed amino acid surface chains containing histidine. The presence of several adjacent histidines such as (His)6-tag increases the affinity to immobilised metal ions. Increasingly, Amintra Ni-NTA and Ni-IDA resins are employed for the purification of histidine-tagged recombinant proteins expressed in bacteria, yeast and mammalian cells.
Amintra Nickel Magnetic.jpg
 
Amintra Nickel Magnetic resin is typically useful in purifying small quantity low concentration histidine-tagged proteins. For example, when 10 – 50 µl of resin is required for protein purification, it is difficult to handle such small quantities in columns. Amintra Nickel Magnetic resins can be easily processed with the aid of a magnet. The liquid / solid separation required in each purification step (e.g. equilibration, binding, washing and elution) is readily achieved with the aid of a magnet (e.g. magnetic stirring bar or other commercial magnetic devices).
 
Key benefits:
  • Tailored base matrix design for batch magnetic purification of proteins
  • High binding capacity (could be over 40 mg/ml)
  • Simple and convenient operation, no chromatography training required
  • Flexible operational conditions
  • Low cost
Characteristics of Amintra Nickel Magnetic
Particle size
50 – 150 µm
Base matrix
Cross-linked 6% agarose encapsulating magnetic particles
Metal ion capacity
Approx. 12 - 25 µmol / ml resin
Binding capacity
Up to 40 mg/ml resin*
Chemical stability
Stable in
0.01M HCl and 1% SDS tested for 30 mins;
0.5 M NaOH tested for overnight;

pH stability
2-14 (<2 h)
4-12 (up to one week)

Storage
20% ethanol at 4oC
Supplied as
50% Slurry



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